Q8IMT2 maps to FlyBase FBgn0051099 and CG31099, with historical CG10551 naming. PMID:32540344(https://pubmed.ncbi.nlm.nih.gov/32540344/), DOI https://doi.org/10.1016/j.ibmb.2020.103429, states: “We propose the hypothesis that members of the arthropod-specific ecdysteroid kinase-like (EcKL) gene family encode detoxicative kinases.” The standard cache is abstract-only. Its broad hypothesis is not evidence for CG31099-specific ecdysteroid 22phosphorylation. The existing TAS and the broader ProtNLM process both remain unresolved; no catalytic loss is alleged. No specific core function is asserted because none has been established. The CHK-domain name is not interpreted as proof of choline kinase substrate specificity.
The selected frozen UniProt record is retained in CG31099-uniprot-source.json, with the complete original prediction metadata in CG31099-predictions-source.json. It maps the exact accession to this FlyBase gene; current sequence identity does not establish the historical predictor input. Gene-level experiments support conserved functions unless a relevant isoform difference is established. Falcon research was requested with perplexity-lite fallback alongside publication caching. Primary publications and sequence observations, rather than AI summaries or ARBA assertions, support the curated conclusions.
The completed Falcon report was inspected. It finds CG31099/Dro14-0 in the 2020 preprint and a preliminary RNAi developmental-lethality phenotype, but no defined reaction. Its biological conclusion agrees with the primary-source uncertainty assessment; the report is not used as an independent assay. PMID:38291829(https://pubmed.ncbi.nlm.nih.gov/38291829/) is now cached with full text. The 2024 phylogenomics study resolves 13 EcKL subfamilies and places the two biochemically characterized ecdysteroid kinases in different subfamilies. Neither CG31099 nor CG10551 is named in its main text. Thus membership of the broad family does not establish a steroid substrate or validate the target kinase without finer evolutionary placement. No specific catalytic loss or inactivity is claimed.
Identity: Q8IMT2 is the full-length 400-aa product of CG31099 (FBgn0051099, EcKL GLC name Dro14-0). UniProt lists CG10551 as GN Name (submitter-imported, ECO:0000313) and GOA carries that symbol; this is a record-level alias, not a different gene model. Not a fragment or isoform: the single SMART CHK domain (130-320) is complete and the catalytic loop H248-S-D250-C-W-V-N-N255 (HxDxxxxN) is retained (CG31099-bioinformatics/motif_scan_rereview.py, RESULTS-rereview.md).
Evolutionary placement (UniProt PANTHER xrefs, 2026-10-20): CG31099 and CG6830 (Q9VGJ8) are PTHR11012:SF6. The two biochemically characterized ecdysteroid 22-kinases are elsewhere: BmEc22K (Q0PCR8) in SF54; AgEcK2, via its direct fly ortholog CG5644 (Q9VSS3), in SF47. Scanlan & Robin 2024 PMID:38291829 name CG5644, CG13813 (SF48) and Pinkman as fly ecdysteroid-kinase candidates; CG31099 is not named. No PAINT/IBA annotations exist for SF6. Interestingly, the FlyBase TAS for GO:0106389 from PMID:32540344 is carried only by the two SF6 members (CG31099 and CG6830) in our repo GOA files, so the curator may have read a subfamily-specific statement in the full text, which we cannot access (journal not OA; bioRxiv blocked).
Decision: GO:0106389 TAS kept UNDECIDED (cannot read the curator's source; evolutionary evidence does not support transfer of the C-22 ecdysteroid specificity). No NEW annotations: generic kinase activity would be a family-level inference with no PAINT support, and the RNAi offspring-reduction phenotype (deep research, from the 2020 preprint) is necessity evidence, not process participation. core_functions left empty. Description rewritten to be project-independent.
Relative: no characterized SF6 member exists. The closest experimentally characterized EcKL by phylogeny is BmEc22K (Bombyx mori, Q0PCR8, SF54), the founding ecdysteroid 22-kinase; it is a family member in a different subfamily, not an ortholog. No review exists in the repo. The SF6 paralog CG6830 has a review (genes/DROME/CG6830) but is uncharacterized. PANTHER family review interpro/panther/PTHR11012/PTHR11012-review.yaml agrees on SF6 placement (CG31099 + CG6830) and scope UNRESOLVED for GO:0106389; no disagreement. No modules cover EcKLs.