Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
SDF-2 induction of terminal differentiation in Dictyostelium discoideum is mediated by the membrane-spanning sensor kinase DhkA.
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DhkA is a membrane-spanning receptor histidine kinase with an extracellular ligand-binding loop and a cytoplasmic catalytic domain that mediates the SDF-2 response.
"By inserting MYC 6 epitopes into DhkA, we were able to show that the loop is extracellular while the catalytic domain is cytoplasmic."
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DhkA autophosphorylates on His1395 in an ATP-dependent manner; this histidine is essential for activity.
"Site-directed mutagenesis of histidine 1395 to glutamine in the catalytic domain blocked autophosphorylation."
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The cAMP phosphodiesterase RegA and PKA act downstream of DhkA.
"Suppressor studies indicate that the cyclic AMP (cAMP) phosphodiesterase RegA and the cAMP-dependent protein kinase PKA act downstream of DhkA."
Genetic interactions of the E3 ubiquitin ligase component FbxA with cyclic AMP metabolism and a histidine kinase signaling pathway during Dictyostelium discoideum development.
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DhkA is a receptor histidine kinase with a CHASE (cytokinin-receptor-like) domain and a histidine kinase domain.
"The DhkA molecule has several domains including a CHASE domain, which was identified as a receptor for plant cytokinins, and a histidine kinase domain"
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dhkA disruption strongly reduces intracellular cAMP levels and suppresses the fbxA sporulation defect.
"The levels of cAMP are relatively constant during multicellular development in all strains except the dhkA mutant, in which it is reduced at least sixfold."
Peptide signaling during terminal differentiation of Dictyostelium.
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SDF-2 is a peptide processed from the acyl-CoA-binding protein AcbA and is the ligand of the DhkA receptor histidine kinase.
"Using a yeast system we show that ligand binding to the SDF-2 receptor histidine kinase, DhkA, inhibits phosphorelay, which can account for its ability to induce rapid sporulation."
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DhkA relays phosphate via RdeA to the RegA response regulator; reduced phosphorelay lowers RegA activity, raising cAMP and activating PKA.
"Reduced phosphorelay to the internal phosphodiesterase RegA via the small H2 protein RdeA results in a decrease in phosphodiesterase activity, leading to accumulation of cAMP and resulting activation of PKA"
High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
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dhkA insertion mutants show developmental phenotypes specific to the slug-to-culmination stage, consistent with a late-development role.
"In contrast, dhkA and acrA show mutant phenotypes only at later stages consistent with their specific roles during slug to culmination stage."
A two-component histidine kinase gene that functions in Dictyostelium development.
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DhkA encodes a two-component histidine kinase with histidine kinase and response regulator domains and functions in prestalk gene regulation and prespore terminal differentiation.
"In Dictyostelium, we found that DhkA functions both in the regulation of prestalk gene expression and in the control of the terminal differentiation of prespore cells."
Histidine kinases in signal transduction pathways of eukaryotes.
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DhkA is a transmembrane histidine kinase whose activation inhibits the RegA cAMP phosphodiesterase, thereby regulating PKA and spore encapsulation.
"Activation of DhkA results in the inhibition of its response regulator, RegA, which is a cAMP phosphodiesterase that regulates the cAMP dependent protein kinase PKA."