Deep research review of CelX (P15329) from Acetivibrio thermocellus
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The protein contains dockerin repeats and EF-hand Ca2+-binding motifs characteristic of cellulosomal enzymes, as well as the Ester_Hydrolysis_Enzymes domain (IPR051532).
"Key Domains: Dockerin_1_rpt. (IPR002105); Dockerin_dom. (IPR016134); Dockerin_dom_sf. (IPR036439); EF_Hand_1_Ca_BS. (IPR018247); Ester_Hydrolysis_Enzymes. (IPR051532)"
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No experimental paper was found that explicitly characterizes CelX/EGX in C. thermocellum. The protein function is inferred from domain architecture.
"No explicit primary literature found that experimentally characterizes a protein named "celX" or "endoglucanase X (EGX)" in C. thermocellum; annotation appears based on sequence/domain evidence and database curation"
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Dockerin-bearing enzymes are secreted and assembled on scaffoldins via Ca2+-dependent cohesin-dockerin binding.
"Dockerin‑bearing CAZymes are secreted and assembled on non‑catalytic scaffoldins via cohesin–dockerin binding; CBMs tether the complex to cellulose... CipA (primary scaffoldin) organizes multiple type‑I cohesins for CAZyme recruitment"
Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity.
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CelX was identified as a secondary ORF in the genomic region encoding CelE, with a C-terminus homologous to other C. thermocellum endoglucanases (the dockerin domain).
"A second ORF which ends 349 bp 5' to the GTG start codon of the celE gene has also been identified. The encoded product contains a C terminus homologous to other C. thermocellum endoglucanases."
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The paper primarily characterizes CelE (endoglucanase E with xylanase activity), not CelX. No experimental characterization of CelX enzymatic activity is provided.
"The complete nucleotide sequence of the Clostridium thermocellum celE gene, coding for an endo-beta-1,4-glucanase (endoglucanase E; EGE) with xylan-hydrolysing activity has been determined."
Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods