Gene Ontology annotation through association of InterPro records with GO terms

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:18755006

Filopodia formation induced by active mDia2/Drf3.

An activated human construct promotes filopodia, while the historical full-length construct occupies the cytosol.

PMID:19457867

Ubiquitin-mediated degradation of the formin mDia2 upon completion of cell division.

Endogenous human DIAPH3 localizes to the cleavage furrow and central spindle during cell division.

PMID:25468996

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.

The assay identifies direct and indirect E-cadherin-neighbor associations and does not establish a purified binary DIAPH3 interface.

Reactome:R-HSA-5666070

RHOB:GTP recruits DIAPH1 or DIAPH3 to endosomes

Reactome:R-HSA-9013009

RHOA binds effectors at the plasma membrane

Reactome:R-HSA-9690493

RHOB binds effectors on endosomes

PMID:16556604

Mechanistic differences in actin bundling activity of two mammalian formins, FRL1 and mDia2.

FH2-containing mDia2 constructs bind and bundle actin, with construct-dependent assembly behavior.

PMID:18458159

The formin mDia2 stabilizes microtubules independently of its actin nucleation activity.

Purified mDia2 fragments bind and stabilize microtubules even when mutations impair their actin-assembly activity.

PMID:19117945

mDia2 shuttles between the nucleus and the cytoplasm through the importin-{alpha}/{beta}- and CRM1-mediated nuclear transport mechanism.

mDia2 has regulated nuclear import and CRM1-dependent export.

PMID:20660154

Rho and anillin-dependent control of mDia2 localization and function in cytokinesis.

Rho and anillin regulate mDia2 recruitment to the cleavage furrow.

PMID:23441200

Diaphanous homolog 3 (Diap3) overexpression causes progressive hearing loss and inner hair cell defects in a transgenic mouse model of human deafness.

Strong mouse Diap3 overexpression causes stereociliary defects and ribbon loss without establishing Diap3 at ribbons or tip-link plaques.

PMID:24069443

The formin protein mDia2 serves as a marker of spindle pole dynamics in vitrified-warmed mouse oocytes.

Endogenous mDia2 colocalizes with spindle-pole and cytoplasmic organizing-center markers in mouse oocytes.

PMID:33036023

Membrane skeleton modulates erythroid proteome remodeling and organelle clearance.

mDia2 supports SRF-dependent Chmp5 expression and ESCRT-associated erythroid remodeling.

PMID:23558171

Nuclear actin network assembly by formins regulates the SRF coactivator MAL.

Formin-dependent nuclear actin assembly regulates the MAL/SRF response.

PMID:25771894

The disease-associated formin INF2/EXC-6 organizes lumen and cell outgrowth during tubulogenesis by regulating F-actin and microtubule cytoskeletons.

EXC-6/INF2-family activity organizes actin and microtubule structures during worm tubulogenesis.

PMID:14992721

The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization.

Construct-dependent FH2 oligomerization alters actin binding and polymerization.

PMID:23034183

RhoD activated by fibroblast growth factor induces cytoneme-like cellular protrusions through mDia3C.

RhoD-specific mDia3C activity should be distinguished from the negative mDia2 control.

PMID:17198702

RhoB and the mammalian Diaphanous-related formin mDia2 in endosome trafficking.

RhoB-associated mDia2 participates in endosomal dynamics.

PMID:15311282

EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration.

The historical construct chain supplies context for mDia-associated microtubule stabilization, without proving identity with a current natural human product.

PMID:20624953

Increased activity of Diaphanous homolog 3 (DIAPH3)/diaphanous causes hearing defects in humans with auditory neuropathy and in Drosophila.

Increased DIAPH3 activity is associated with auditory neuropathy.

file:human/DIAPH3/DIAPH3-source-guide.md

DIAPH3 source and construct guide

Public original locators, assay and species limits, ancestral-node provenance and exact Reactome chains are recorded together.