Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
A novel quality control compartment derived from the endoplasmic reticulum.
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When expressed without ASGR1 in heterologous cells, the precursor of human ASGR2 isoform H2a accumulates in a juxtanuclear pre-Golgi endoplasmic reticulum quality control compartment prior to ubiquitin-proteasome ERAD.
"We found that substrates of this pathway, the precursor of human asialoglycoprotein receptor H2a and free heavy chains of murine class I major histocompatibility complex (MHC), accumulate in a novel preGolgi compartment that is adjacent to but not overlapping with the centrosome, the Golgi complex, and the ER-to-Golgi intermediate compartment (ERGIC)."
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During endoplasmic reticulum-associated degradation, unassembled H2a associates with the ER translocon subunit Sec61.
"On its way to degradation, H2a associated increasingly after synthesis with the ER translocon Sec61."
Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1.
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Yeast two-hybrid screening and GST pull-down assays identified interactions between LASS2 (CERS2) and membrane-associated receptors or transporters.
"Yeast two-hybrid screening and glutathione S-transferase pull-down assays showed that the LASS2 protein interacts with several membrane-associated receptors or transporters."
Nonpalmitoylated human asialoglycoprotein receptors recycle constitutively but are defective in coated pit-mediated endocytosis, dissociation, and delivery of ligand to lysosomes.
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The human hepatic asialoglycoprotein receptor contains subunits H1 (ASGR1) and H2 (ASGR2) and internalizes desialylated glycoproteins via the clathrin-coated pit pathway for lysosomal degradation.
"The hepatic asialoglycoprotein receptor (ASGP-R) internalizes desialylated glycoproteins via the clathrin-coated pit pathway and mediates their delivery to lysosomes for degradation. The human ASGP-R contains two subunits, H1 and H2."
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Palmitoylation of the asialoglycoprotein receptor subunits is required for efficient clathrin-dependent endocytosis, ligand dissociation, and delivery to lysosomes.
"We conclude that palmitoylation of the ASGP-R is required for its efficient endocytosis of ligand by the clathrin-dependent endocytic pathway and, in particular, for the proper dissociation and delivery of ligand to lysosomes."
Toward an understanding of the protein interaction network of the human liver.
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High-throughput yeast two-hybrid screen of human liver proteins mapping binary protein-protein interactions.
"In this study, we map the interactions of an unbiased selection of 5026 human liver expression proteins by yeast two-hybrid technology and establish a human liver protein interaction network (HLPN) composed of 3484 interactions among 2582 proteins."
Asialoglycoprotein receptor 1 mediates productive uptake of N-acetylgalactosamine-conjugated and unconjugated phosphorothioate antisense oligonucleotides into liver hepatocytes.
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Co-expression of ASGR1 incorporates ASGR2 into ASGR1/ASGR2 heterodimers that are exported from the ER and targeted to the hepatocyte plasma membrane.
"This data parallels previous reports demonstrating that co-expression of ASGR1 results in ASGR2 incorporation into ASGR1/ASGR2 heterodimers that are then exported from the ER and are ultimately targeted to the plasma membrane"
Sequence of a second human asialoglycoprotein receptor: conservation of two receptor genes during evolution.
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Identification and cDNA cloning of the second human hepatic asialoglycoprotein receptor subunit H2 (ASGR2), which shares 58% protein sequence homology with H1 (ASGR1).
"From the same library, we have isolated and sequenced a clone encoding a second ASGP receptor, H2, with a protein sequence homology of 58% to H1."
PALM-C36-ASGR1:PALM-C54,58-ASGR2 binds desialated glycoproteins
UniProtKB record for human ASGR2 (P07307)
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ASGR2 mediates the endocytosis of desialylated plasma glycoproteins by recognizing terminal galactose and N-acetylgalactosamine units.
"Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units."
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ASGR2 is a single-pass type II membrane protein.
"Membrane; Single-pass type II membrane protein."
Research and curation notes for human ASGR2