GALNT3 evidence from the original vesicle-fraction study

This page records a selected target-table excerpt and its identification chain. It supplements the abstract-only PMID:18570454 cache; it is not a replacement full-text cache.

Original source: DOI 10.1021/pr800225z, author-hosted PDF. The retained PDF is 1,005,190 bytes, SHA256 7d3b945b98a6696ca6146956da71378304b2de9f40867ee86729248a395372fc.

Target row and identifier

The following line is transcribed from Table 2, printed page 3478, preserving the extracted spacing:

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase       gi|1617312     72638.4             1                     v

The table columns give a predicted mass of 72,638.4 Da, one matched peptide, and a mark in fraction 3. The table uses a generic enzyme name; target identity comes from its accession. The official NCBI CAA63371.1 protein record, retrieved as GenPept for GI1617312, identifies human GalNAc-T3 and cross-references HGNC:4125 and UniProtKB/Swiss-Prot:Q14435. This is an accession mapping, not a peptide-spectrum reassignment or a modern isoform assignment. The retained GenPept response is 4,171 bytes, SHA256 e63151030f275ab9164a31ce94a8cbc361a41ce1cfc2ce7c2fada8bd099fa406.

Selected experimental scope

Methods/Results and Tables 1–2 describe HB1.F3 conditioned-medium fractionation, flow field-flow separation, TEM characterization and LC-MS/MS. Table 1, printed page 3477, gives fraction-3 particle diameter 34.0 ± 1.40 nm for 16 particles. Results on printed page 3480 state a Mascot-score threshold above 50 and detection in more than two of three shotgun analyses for single-peptide identifications. These criteria concern technical repetition; they do not establish independent biological replication.

The selected original text supports GALNT3 association with the characterized vesicle fraction. The single matched peptide and possible co-isolated material limit the interpretation. No membrane orientation, extracellular catalytic activity, vesicle-biogenesis role or current-product assignment is inferred. This review did not reanalyze the raw spectra or independently inspect the TEM images. The retained text extraction is 47,689 bytes, SHA256 5ad89e30471bf4f868ad0dbc067f4a88243a381c24412efcf5906756fbb7ccfc.