Annotation inferences using phylogenetic trees
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The sigma factor activity IBA derives from PAINT node PTN001249270 in PTHR30173, seeded by M. tuberculosis SigJ (L0TCG5, subfamily SF36); Q9KZ33 is in subfamily SF43, so the node lies above both subfamilies.
Automatic assignment of GO terms using logical inference, based on inter-ontology links
Deep research summary for Q9KZ33/SCO7099
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SCO7099 is a predicted ECF sigma factor with sigma2 and sigma4 domain architecture
"The presence of ECF_Sigma-70_Domain and RNA_pol_sigma_r3/r4-like domains strongly supports classification as a bona fide sigma factor capable of directing transcription initiation"
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SCO7099 remains functionally uncharacterized with no defined regulon
"For SCO7099, the actual promoter consensus sequence, target genes, and regulon composition remain completely unknown"
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Overexpression of SCO7099 failed in a systematic study
"Transformants were not obtained for three sigma factors: SCO4908 (SigQ), SCO5243 (SigH), and SCO7099"
UniProtKB record Q9KZ33 (SCO7099)
Q9KZ33 comparison with M. tuberculosis ECF41 sigma factors
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Q9KZ33 aligns over ~270 of 300 residues, including the C-terminal extension, to both M. tuberculosis SigI (SF43) and SigJ (SF36) at ~33-35% identity; pairwise identity does not discriminate between the two subfamilies.
"Pairwise identity does not discriminate SigI from SigJ (both ~33-35%)"
Extracytoplasmic function σ factors of the widely distributed group ECF41 contain a fused regulatory domain.
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ECF41 sigma factors carry a C-terminal extension required for sigma activity and lack separate anti-sigma factors.
"They lack obvious anti-σ factors that typically control activity of other ECF σ factors"
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Streptomyces genomes encode several ECF41 sigma factors.
"Especially the genus Streptomyces contains large numbers of ECF41 σ factors with at least four copies per genome"
Crystallographic studies of the extracytoplasmic function σ factor σ(J) from Mycobacterium tuberculosis.
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M. tuberculosis SigI and SigJ are ECF sigma factors without an associated anti-sigma factor, carrying a conserved C-terminal extension.
"Among ECF σ factors, σ C , σ G , σ I and σ J are not associated with an anti-σ factor."
The C-terminal domain of M. tuberculosis ECF sigma factor I (SigI) interferes in SigI-RNAP interaction.
Machine-Learning Analysis of Streptomyces coelicolor Transcriptomes Reveals a Transcription Regulatory Network Encompassing Biosynthetic Gene Clusters.
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No transformants were obtained for SCO7099 overexpression; its function is listed as unknown.
"Transformants were not obtained for three sigma factors: SCO4908 (SigQ; related to secondary metabolism and morphological development),[ 42 ] SCO5243 (SigH; related to morphological development and osmotic stress response),[ 43 ] and SCO7099 (unknown function)"