Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by ARBA machine learning models
A Ycf2-FtsHi Heteromeric AAA-ATPase Complex Is Required for Chloroplast Protein Import.
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Arabidopsis biochemistry identifies FTSH12 as a constituent of the 2-MD Ycf2/FtsHi import motor, which associates with TIC and translocating preproteins.
"a 2-MD heteromeric AAA-ATPase complex associates with the TIC complex and functions as the import motor, directly interacting with various translocating preproteins"
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FTSH12 is the only complex subunit that keeps the FtsH zinc-binding active site.
"only FtsH12 contains the zinc binding active site generally conserved among FtsH-type metalloproteases"
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H769Y complementation preserves viability and import, separating the essential motor role from zinc-dependent proteolysis.
"even the FtsH12 zinc binding site is dispensable for its essential function"
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FTSH12 lacks the pore-loop hydrophobic residues that grip substrates, and it cross-links only weakly to translocating preproteins.
"We also detected trace amounts of FtsH12 in the pull-down fraction"
Abundance of metalloprotease FtsH12 modulates chloroplast development in Arabidopsis thaliana.
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Fractionation and protease-protection experiments place FTSH12 in the inner envelope, with its ATPase and protease domains facing the stroma.
"FtsH12 is located in the inner chloroplast envelope, with the C-terminal part containing the AAA-type domain and the catalytic domain facing the stroma"
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Proteome and N-terminome analyses do not support FTSH12 as the general maturase for imported proteins.
"N-terminome analyses further demonstrated normal proteolytic maturation of"
Exact-input alignment of A0A1P8ARD2 to the Q9SAJ3 same-gene reference with feature mapping
Frozen exact-input ProtNLM output and UniProt sequence for A0A1P8ARD2
FTSH12 gene-model comparison (re-review)
Structural insights into the chloroplast protein import in land plants.
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The 2024 Arabidopsis structural work confirms the heterohexameric AAA+ ATPase motor architecture of Ycf2-FtsHi. It does not establish an FTSH12 protease substrate.
"The Ycf2-FtsHi structure reveals a heterohexameric AAA+ ATPase motor module with characteristic features."