Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.
Nijmegen breakage syndrome disease protein and MRE11 at PML nuclear bodies and meiotic telomeres.
Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres.
Chk2 activation dependence on Nbs1 after DNA damage.
Rescue of a telomere length defect of Nijmegen breakage syndrome cells requires NBS and telomerase catalytic subunit.
Mre11 complex and DNA replication: linkage to E2F and sites of DNA synthesis.
NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain.
The mismatch repair system is required for S-phase checkpoint activation.
Recruitment of NBS1 into PML oncogenic domains via interaction with SP100 protein.
Critical role for mouse Hus1 in an S-phase DNA damage cell cycle checkpoint.
MDC1 is a mediator of the mammalian DNA damage checkpoint.
ATM activation by DNA double-strand breaks through the Mre11-Rad50-Nbs1 complex.
ATM-dependent phosphorylation of ATF2 is required for the DNA damage response.
The involvement of the Mre11/Rad50/Nbs1 complex in the generation of G-overhangs at human telomeres.
MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks.
Multifactorial contributions to an acute DNA damage response by BRCA1/BARD1-containing complexes.
Two-step activation of ATM by DNA and the Mre11-Rad50-Nbs1 complex.
PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex.
SIRT1 regulates the function of the Nijmegen breakage syndrome protein.
MRE11-RAD50-NBS1 and ATM function as co-mediators of TRF1 in telomere length control.
RNF8 ubiquitylates histones at DNA double-strand breaks and promotes assembly of repair proteins.
RNF8 transduces the DNA-damage signal via histone ubiquitylation and checkpoint protein assembly.
Cell cycle-dependent complex formation of BRCA1.CtIP.MRN is important for DNA double-strand break repair.
Phosphorylation of SDT repeats in the MDC1 N terminus triggers retention of NBS1 at the DNA damage-modified chromatin.
Structure of a second BRCT domain identified in the nijmegen breakage syndrome protein Nbs1 and its function in an MDC1-dependent localization of Nbs1 to DNA damage sites.
Phospho-dependent interactions between NBS1 and MDC1 mediate chromatin retention of the MRN complex at sites of DNA damage.
MDC1 regulates intra-S-phase checkpoint by targeting NBS1 to DNA double-strand breaks.
Purification of proteins associated with specific genomic Loci.
RAD50 and NBS1 form a stable complex functional in DNA binding and tethering.
Histone H2AX participates the DNA damage-induced ATM activation through interaction with NBS1.
MRE11-RAD50-NBS1 is a critical regulator of FANCD2 stability and function during DNA double-strand break repair.
SOSS complexes participate in the maintenance of genomic stability.
N terminus of CtIP is critical for homologous recombination-mediated double-strand break repair.
A supramodular FHA/BRCT-repeat architecture mediates Nbs1 adaptor function in response to DNA damage.
A divalent FHA/BRCT-binding mechanism couples the MRE11-RAD50-NBS1 complex to damaged chromatin.
Acetyltransferase p300 regulates NBS1-mediated DNA damage response.
Crystal structure of human Mre11: understanding tumorigenic mutations.
Five dysfunctional telomeres predict onset of senescence in human cells.
BRCA1 tumor suppressor network: focusing on its tail.
Skp2 E3 ligase integrates ATM activation and homologous recombination repair by ubiquitinating NBS1.
CDK targeting of NBS1 promotes DNA-end resection, replication restart and homologous recombination.
The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination.
Rad17 recruits the MRE11-RAD50-NBS1 complex to regulate the cellular response to DNA double-strand breaks.
ATRX dysfunction induces replication defects in primary mouse cells.
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage.
CtIP tetramer assembly is required for DNA-end resection and repair.
Notch is a direct negative regulator of the DNA-damage response.
MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.
Acetylation of Histone H2AX at Lys 5 by the TIP60 Histone Acetyltransferase Complex Is Essential for the Dynamic Binding of NBS1 to Damaged Chromatin.
ATM-Dependent Phosphorylation of All Three Members of the MRN Complex: From Sensor to Adaptor.
VRK1 phosphorylates and protects NBS1 from ubiquitination and proteasomal degradation in response to DNA damage.
MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.
Nbs1 Converts the Human Mre11/Rad50 Nuclease Complex into an Endo/Exonuclease Machine Specific for Protein-DNA Adducts.
Phosphorylated CtIP Functions as a Co-factor of the MRE11-RAD50-NBS1 Endonuclease in DNA End Resection.
A balance between elongation and trimming regulates telomere stability in stem cells.
NBS1 Phosphorylation Status Dictates Repair Choice of Dysfunctional Telomeres.
Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break Repair.
DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity.
The MRE11-RAD50-NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair.
CtIP-BRCA1 complex and MRE11 maintain replication forks in the presence of chain terminating nucleoside analogs.
NBS1 promotes the endonuclease activity of the MRE11-RAD50 complex by sensing CtIP phosphorylation.
Pellino1 regulates reversible ATM activation via NBS1 ubiquitination at DNA double-strand breaks.
Purification and Biophysical Characterization of the Mre11-Rad50-Nbs1 Complex.
MRE11-RAD50-NBS1 promotes Fanconi Anemia R-loop suppression at transcription-replication conflicts.
NBS1 lactylation is required for efficient DNA repair and chemotherapy resistance.
Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome.
The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response.
MRN complex binds DNA double strand breaks
MRN complex bound to shortened telomeres recruits ATM
MRN complex binds shortened telomeres
MRN bound to shortened telomeres activates ATM
KAT5 acetylates ATM at DNA DSBs
HERC2 and PIAS4 are recruited to DNA DSBs
RNF8 binds phosphorylated MDC1 at DNA DSBs
PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs
HERC2 facilitates UBE2N:UBE2V2 binding to RNF8
RNF8 and RNF168 ubiquitinate H2AFX
WHSC1 dimethylates histone H4 on lysine K21 at DSBs
RNF8 and RNF168 ubiquitinate KDM4A,B
UIMC1 and FAM175A bind DNA DSBs
Formation of BRCA1-A complex at DNA DSBs
PPP5C dephosphorylates TP53BP1
ATM phosphorylates TP53BP1 at DNA DSBs
CHEK2 is recruited to DNA DSBs
CHEK2 phosphorylates BRCA1
ATM phosphorylates EYA1-4
EYA1-4 dephosphorylates tyrosine Y142 of H2AFX
APBB1 and MAPK8 bind diphosphorylated H2AFX
KPNA2 translocates NBN to the nucleus
MRN complex binds CDK2 and RBBP8
CDK2 phosphorylates RBBP8
BRCA1 binds phosphorylated RBBP8
Binding of ATR:ATRIP to RPA at resected DNA DSBs
CHEK1 is recruited to resected DNA DSBs
Activation of CHEK1 at resected DNA DSBs
ATR activation at DNA DSBs
BCDX2 complex stabilizes RAD51 filament
CX3 complex binds D-loop structures
EXO1 or DNA2 in complex with BLM or WRN binds initially resected DNA DSBs along with BRIP1 recruitment
Long-range resection of DNA DSBs by EXO1 or DNA2
BLM mediates dissolution of double Holliday junction
MUS81:EME1,EME2 cleaves D-loop
Resolution of D-loops cleaved by MUS81:EME1 or MUS81:EME2
Resolution of Holliday junctions cleaved by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2)
RAD52 promotes single strand annealing at resected DNA DSBs
ERCC1:XPF cleaves flaps generated by SSA
RIF1 and PAX1IP bind TP53BP1 at DNA DSBs
Activated ATM phosphorylates DCLRE1C
TP53BP1 recruits DCLRE1C to ATM
MRN and RBBP8 resect DNA DSBs in MMEJ
PARP1 or PARP2, FEN1 and POLQ are recruited to MMEJ site
POLQ extends annealed 3'-ssDNA overhangs in MMEJ
PARP1,PARP2 dimers bound to MMEJ sites autoPARylate
FEN1 cleaves displaced ssDNA flaps during MMEJ
MRN recruits LIG3:XRCC1 to MMEJ sites
LIG3 ligates remaining SSBs in MMEJ
Ligation of DNA and formation of Holliday structures following repair synthesis
Association of RPA complexes with ssDNA at resected DNA DSBs
ATM phosphorylates histone H2AFX on S139 at DNA DSBs
Phosphorylation of BRCA1-A complex at multiple sites by ATM
RAD51 binds BRCA2 at resected DNA DSBs
Association of RAD51 with RAD52:DNA double-strand break ends
TP53BP1 associates with H4K20Me2 at DNA DSBs
Association of RAD52 with the RPA complex at resected DNA DSBs
MDC1 associates with gamma-H2AFX at nuclear foci
Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2)
D-loop dissociation and strand annealing
D-loop extension by DNA polymerases
Association of Ku heterodimer with ends of DNA double-strand break
ATM recognizes H2AFX-Nucleosomes
Initial resection of double-strand break ends
MRN complex bound to DNA ends recruits ATM
D-loop formation mediated by PALB2, BRCA2 and RAD51
KAT5 acetylates ATM at shortened telomeres
Phosphorylation and activation of CHEK2 by ATM
Association of RAD50:MRE11A complex with NBN (NBS1) via MRE11A interaction
Defective D-loop formation mediated by PALB2, BRCA2 and RAD51 due to loss-of-function of BRCA1 in PALB2 binding
Defective D-loop formation mediated by PALB2, BRCA2 and RAD51 due to loss-of-function of PALB2 in BRCA1 binding
Defective D-loop formation mediated by PALB2, BRCA2 and RAD51 due to loss-of-function of PALB2 in binding to BRCA2/RAD51/RAD51C
BRCA2 mutants with BRC defects or a defect in the C-terminal RAD51 binding site do not bind RAD51
Defective recruitment of BRCA2 and RAD51 due to loss of BRCA2 function in PALB2 binding
ERCC1:XPF binds DNA DSBs with annealed 3' ssDNA overhangs and displaced flaps generated by SSA