Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins.
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hCRY1 and hCRY2 contain FAD and pterin cofactors but lack photolyase activity on cyclobutane pyrimidine dimers and (6-4) photoproducts.
"were found to contain FAD and a pterin cofactor. Like the plant blue-light photoreceptors, both hCRY1 and hCRY2 lacked photolyase activity on the cyclobutane pyrimidine dimer and the (6-4) photoproduct"
Human blue-light photoreceptor hCRY2 specifically interacts with protein serine/threonine phosphatase 5 and modulates its activity.
Molecular cloning of a second human stanniocalcin homologue (STC2).
Light-independent role of CRY1 and CRY2 in the mammalian circadian clock.
Dec1 and Dec2 are regulators of the mammalian molecular clock.
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Cry proteins act together with Per proteins to inhibit Per transcription and close the autoregulatory feedback loop.
"Protein products of Per act together with Cry proteins to inhibit Per transcription, thus closing the autoregulatory feedback loop"
Purification and properties of human blue-light photoreceptor cryptochrome 2.
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Purified hCRY2 binds dsDNA weakly and ssDNA with higher affinity, stimulated by (6-4) photoproducts. CRY2 lacks photorepair activity.
"binds to double-stranded DNA weakly and to single-stranded DNA with higher affinity, and this binding is further stimulated by the presence of a (6-4) photoproduct"
A novel autofeedback loop of Dec1 transcription involved in circadian rhythm regulation.
Expression of the gene for Dec2, a basic helix-loop-helix transcription factor, is regulated by a molecular clock system.
Role of structural plasticity in signal transduction by the cryptochrome blue-light photoreceptor.
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Light-dependent conformational change demonstrated for Arabidopsis Cry1, not human CRY2. The paper characterizes CRY structure but does not demonstrate light response function for human CRY2.
"we demonstrate a light-dependent conformational change in the C-terminal domain of Arabidopsis Cry1"
Posttranslational regulation of the mammalian circadian clock by cryptochrome and protein phosphatase 5.
SCFFbxl3 controls the oscillation of the circadian clock by directing the degradation of cryptochrome proteins.
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CRY1 and CRY2 are ubiquitinated and degraded by SCF(FBXL3), which is essential for clock oscillation.
"both Cry1 and Cry2 proteins are ubiquitinated and degraded via the SCF(Fbxl3) ubiquitin ligase complex"
Identification of two amino acids in the C-terminal domain of mouse CRY2 essential for PER2 interaction.
Cryptochromes mediate rhythmic repression of the glucocorticoid receptor.
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CRY1 and CRY2 interact with the glucocorticoid receptor in a ligand-dependent manner, globally altering glucocorticoid transcriptional response. Cryptochrome deficiency causes glucose intolerance and constitutively high corticosterone.
"cryptochromes 1 and 2, interact with the glucocorticoid receptor in a ligand-dependent fashion and globally alter the transcriptional response to glucocorticoids"
A proteome-scale map of the human interactome network.
Nuclear receptor HNF4A transrepresses CLOCK:BMAL1 and modulates tissue-specific circadian networks.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.