Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Prenylated prelamin A interacts with Narf, a novel nuclear protein.
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Human NARF constructs localize to nuclei and bind prenylated prelamin A; the paper does not establish activity of the selected 217-residue product.
"When a FLAG epitope-tagged Narf is expressed
in HeLa cells, it is exclusively nuclear and partially co-localizes with the
nuclear lamina."
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NARF binding to the prelamin A tail requires prelamin A farnesylation and its CaaX motif.
"The farnesylation status of prelamin A determines its ability to bind to Narf."
A role for IOP1 in mammalian cytosolic iron-sulfur protein biogenesis.
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Compared with IOP1 depletion, IOP2/NARF depletion did not impair cytosolic or mitochondrial aconitase activity, distinguishing the paralogs in this tested CIA phenotype.
"Knockdown of IOP2, in contrast, had no
effect on either."
NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification.
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In hypoxic human breast cancer cells, NARF is a HIF-1 target that acts as a coactivator recruiting KDM6A to OCT4-bound pluripotency genes.
"NARF functions as an essential coactivator by recruiting the histone demethylase KDM6A to OCT4 bound to genes encoding the pluripotency factors NANOG, KLF4, and SOX2"
Exact NARF product-to-reference sequence mapping
NARF J3KS48 vs canonical Q9UHQ1 independent re-review mapping
Falcon deep research report on human NARF
Frozen exact-input ProtNLM output and UniProt sequence for J3KS48