Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7.
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SCAMP1 interacts with NHE7 at the TGN and recycling vesicles
"The majority of the NHE7-SCAMP complexes accumulated at the TGN, but a minor fraction also resided in recycling vesicles"
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SCAMP1 regulates shuttling of NHE7 between compartments
"We propose a model wherein SCAMPs participate in the shuttling of NHE7 between recycling vesicles and the TGN"
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Interaction is mediated by the conserved TM2-TM3 cytoplasmic loop
"the C-terminal cytoplasmic tail of NHE7 bound preferentially to a highly conserved cytoplasmic loop between the second and the third transmembrane segments (TM2-TM3 loop) of SCAMP2"
A proteome-scale map of the human interactome network.
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High-throughput binary protein-protein interaction mapping
"Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions"
Architecture of the human interactome defines protein communities and disease networks.
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Interactome mapping study
"BioPlex 2.0 contains more than 29,000 previously unknown co-associations and provides functional insights into hundreds of poorly characterized proteins"
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
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Neurodegenerative disease protein interaction network
"an interactome map that focuses on neurodegenerative disease (ND), connects ∼5,000 human proteins via ∼30,000 candidate interactions"
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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Cell-type-specific interactome analysis
"we have created two proteome-scale, cell-line-specific interaction networks"
CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system.
Differential CFTR-Interactome Proximity Labeling Procedures Identify Enrichment in Multiple SLC Transporters.
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CFTR proximity labeling identifies trafficking factors
"The two proximity labeling approaches identified both known and additional CFTR protein partners, including multiple SLC transporters"
Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions.
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SCAMP1-3 are products of distinct genes
"The structures indicated that SCAMPs are highly related products of distinct genes"
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SCAMPs have similar subcellular distributions in post-Golgi compartments
"Examination of the codistribution of the three forms within individual cells using double label immunofluorescence indicates extensive colocalization"
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SCAMPs function together at same sites during vesicular transport
"SCAMPs may largely function at the same sites during vesicular transport rather than in separate post-Golgi recycling pathways"
Exocytosis of specific granule membrane proteins
Deep research review of SCAMP1 literature
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SCAMP family structural and functional analysis from multiple studies
"NPF motifs recruit EH-domain proteins (Eps15/intersectin), and E peptide is conserved and membrane-active"