Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
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IBA annotations for TORC2 complex, TORC2 signaling, cytoplasm, plasma membrane, and PIP2 binding are phylogenetically inferred from characterized SIN1 family orthologs
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Combined Automated Annotation using Multiple IEA Methods
Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity.
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Sin1 is essential for TORC2 complex formation, stability, and kinase activity
"Sin1 is an essential component of TORC2 but not of TORC1, and functions similarly to Rictor, the defining member of TORC2, in complex formation and kinase activity"
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Sin1 knockdown reduces Akt phosphorylation in Drosophila cells and in vivo
"Knockdown of Sin1decreases Akt phosphorylation in both Drosophila and mammalian cells and diminishes Akt function in vivo"
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Sin1 is required for Rictor-mTOR interaction
"It also disrupts the interaction between Rictor and mTOR"
Re-evaluating AKT regulation: role of TOR complex 2 in tissue growth.
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Drosophila sin1 mutants show strongly reduced Akt hydrophobic motif phosphorylation
"Mutants removing critical TORC2 components, rictor and sin1, strongly reduced AKT hydrophobic motif (HM) phosphorylation and AKT activity"
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Despite reduced Akt activity, sin1 mutants show only minor growth impairment under normal conditions
"showed only minor growth impairment"
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TORC2 acts as a rheostat amplifying Akt signaling at high pathway activity
"TORC2 acts as a rheostat to broaden the range of AKT signaling at the high end of its range"
The target of rapamycin complex 2 controls dendritic tiling of Drosophila sensory neurons through the Tricornered kinase signalling pathway.
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Sin1, Rictor, and TOR (TORC2 components) are required for dendritic tiling in class IV da neurons
"Sin1, Rictor, and target of rapamycin (TOR), components of the TOR complex 2 (TORC2), are required for dendritic tiling of class IV da neurons"
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TORC2 phosphorylates Tricornered (NDR kinase) to regulate tiling
"TORC2 is essential for Trc phosphorylation"
Diet and energy-sensing inputs affect TorC1-mediated axon misrouting but not TorC2-directed synapse growth in a Drosophila model of tuberous sclerosis.
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Rheb-mediated synapse overgrowth is rescued by loss of sin1 and rictor (TORC2) but not TORC1 components
"Rheb-mediated synapse overgrowth was not rescued by knockdown of TorC1 signaling, however it was rescued by loss of the TorC2 components sin1 and rictor"
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TORC2 directs synapse growth independently of TORC1
"synapse overgrowth was only suppressed by reducing the function of Tor complex 2 (TorC2) components"
LST8 regulates cell growth via target-of-rapamycin complex 2 (TORC2).
TORC2 mediates the heat stress response in Drosophila by promoting the formation of stress granules.
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Sin1 and Rictor mutants are heat sensitive
"Rictor and Sin1 homozygous mutants are heat sensitive"
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TORC2 is required for stress granule formation under heat stress
"TORC2 is specifically required for stress granule formation under heat stress"
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S505 Akt phosphorylation is absent in Sin1 mutants
"S505 phosphorylation was nearly absent in Sin1 mutants"
Deep research summary for dSin1 function and TORC2 signaling
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Sin1 PH domain mediates membrane/phosphoinositide binding for TORC2 localization
"PH domain: membrane/phosphoinositide binding; CRIM: acidic protrusion that recruits AGC substrates"
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mTORC2/Sin1 pools exist at plasma membrane and other membrane compartments
"mTORC2/Sin1 pools reported at plasma membrane, mitochondria-associated membranes, endosomes and vesicles"