Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Feedback regulation of the MBF transcription factor by cyclin Cig2.
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MBF contains Res1 and Res2, binds MCB elements and activates transcription of genes required for S phase; cig2 is an MBF target.
"The Mlu1-binding factor (MBF) from the fission yeast Schizosaccharomyces pombe contains the proteins Res1p and Res2p and binds to the Mlu1 cell-cycle box (MCB) element in DNA, activating the transcription of genes required for S phase."
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Cig2 binds Res2, promotes phosphorylation of Res1 and inhibits MBF-dependent transcription, forming a feedback-inhibition loop.
"Cig2p can bind to Res2p, promote the phosphorylation of Res1p and inhibit MBF-dependent gene transcription."
DSC1-MCB regulation of meiotic transcription in Schizosaccharomyces pombe.
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cdc10, res2, rep1 and rep2 are required for correct meiotic MCB-driven transcription, whereas res1 is not.
"We found that cdc10+, res2+, rep1+ and rep2+ are required for correct meiotic transcription, while res1+ is not required for this process."
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rec8, rec11, cdc18 and cdc22 carry MCB motifs and are co-ordinately transcribed in premeiotic S phase, and a DSC1-like MCB-binding complex is present in meiotic cells.
"A DSC1-like transcription factor complex that binds to MCB motifs was also identified in meiotic cells."
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination.
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ctp1 transcription is periodic with onset at the start of DNA replication, the signature of an MBF target.
"Transcription of ctp1(+) is periodic during the cell cycle, with the onset of its expression coinciding with the start of DNA replication."
DNA replication checkpoint promotes G1-S transcription by inactivating the MBF repressor Nrm1.
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Nrm1 is an MBF-specific corepressor of G1/S transcription and a Cds1 target whose phosphorylation releases it from MBF under replication stress.
"Nrm1, a corepressor of MBF dependent G 1 -S transcription, is a target of the DNA replication checkpoint kinase Cds1"
Yox1 links MBF-dependent transcription to completion of DNA synthesis.
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Purification of native MBF through Cdc10-HA recovers Cdc10, Res1 and Res2 as the most enriched proteins together with the repressor Yox1.
"The most enriched proteins in the purification were Cdc10, Res1, Res2, as expected"
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Yox1 binding to Cdc10 requires an intact MBF complex including Res2.
"in the absence of Res1 or Res2, Yox1 was not able to bind to Cdc10"
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MBF remains bound to its target promoters throughout the cell cycle, so its activity is not regulated by DNA-binding modulation.
"MBF is bound to its target promoters throughout the cell cycle ( Wuarin et al, 2002 ), suggesting that MBF activity is not due to modulation of its DNA-binding activity."
The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor.
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The MBF core is Cdc10 with Res1 and Res2, which form a heterodimeric DNA-binding domain; the DNA-binding activity of the complex resides in the Res2 N-terminal region.
"Res2, which contains the DNA-binding activity of the MBF complex in its amino-terminal region"
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Res2-HA is loaded on the cdc22 and cdc18 promoters by ChIP and is released from chromatin, together with Res1, after MMS-induced DNA damage, while the Cdc10-Res2 interaction is preserved.
"both Res1 and Res2 are released from chromatin after treatment with MMS"
Cell Cycle-Regulated Transcription of CENP-A by the MBF Complex Ensures Optimal Level of CENP-A for Centromere Formation.
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A visual screen for CENP-A misregulation recovered nrm1, yox1 and res2 deletions; Res2 binds the cnp1 promoter by ChIP and res2 deletion raises cnp1 mRNA about twofold.
"ChIP analysis showed that Nrm1, Yox1, and Res2 bind to the cnp1 promoter ( Figure 4B )."
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Nrm1 and Yox1 repress cnp1 through Res2, and Res2 has both positive and negative roles in MBF-mediated transcription.
"Deletion of res2 has a weaker effect on CENP-A transcription and chromosome segregation, likely resulting from the fact that Res2 has both negative and positive roles in MBF-mediated transcription."
A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Fission yeast Rep2 is a putative transcriptional activator subunit for the cell cycle 'start' function of Res2-Cdc10.
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Rep2 is a transcriptional activator subunit that interacts with the MCB-binding subunit complex formed by Res2 and Cdc10.
"Our data suggest that Rep2 is a transcriptional activator subunit which interacts with the MCB binding subunit complex formed by Res2 and Cdc10."
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Res1-Cdc10 and Res2-Cdc10 are two overlapping Start systems acting in both the mitotic and meiotic cycles.
"Fission yeast possess two such overlapping systems, Res1-Cdc10 and Res2-Cdc10, both of which act to start the mitotic and meiotic cycles."
A B-type cyclin negatively regulates conjugation via interacting with cell cycle 'start' genes in fission yeast.
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The conjugation-induced poly(A)- cyc17 (cig2) transcript depends on res2, whereas the vegetative poly(A)+ transcript is reduced in res1 cells.
"This induction is lost in res2- cells, whereas the poly(A)+ transcript is significantly reduced in res1- cells."
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Mating inhibition and pat1 rescue by res1+/res2+ overexpression require cyc17.
"However, the mating inhibition as well as the ability to rescue the pat1 mutation by overexpression of res1+ and res2+ are totally abolished in cyc17- cells."
pct1+, which encodes a new DNA-binding partner of p85cdc10, is required for meiosis in the fission yeast Schizosaccharomyces pombe.
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pct1 (res2) encodes a 73 kDa protein that forms an MCB-binding heteromer with p85cdc10; the complex binds MCB but not SCB or E2F sites in vitro and in vivo.
"The p73pct1/p85cdc10 complex binds both in vitro and in vivo to MCB but not SCB or E2F sites."
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pct1 deletion is viable but causes a severe meiotic defect; pct1 overexpression rescues cdc10-129 but not a cdc10 null.
"A deletion of pct1+ is not lethal but does result in a severe meiotic defect."
res2+, a new member of the cdc10+/SWI4 family, controls the 'start' of mitotic and meiotic cycles in fission yeast.
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res2+ was isolated as a multicopy suppressor of a res1 null mutant and encodes a 73 kDa Swi/ankyrin protein most similar to Res1.
"res2+ has been isolated as a multicopy suppressor of a res1- null mutant and specifies a 73 kDa protein, which has two copies of the Swi/ankyrin motif and shares the highest sequence and structure similarity with the Res1 protein."
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res2+ is largely redundant with res1+, required for initiation of mitotic and premeiotic DNA synthesis, has an additional role in meiotic division, is induced during conjugation and depends on cdc10+.
"res2+ is largely redundant in function with res1+ and is required for the initiation of mitotic and premeiotic DNA synthesis, but has an additional role in meiotic division."
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Res1-Cdc10 plays the major role in the mitotic cycle and Res2-Cdc10 in the meiotic cycle.
"We conclude that the fission yeast contains two functionally overlapping parallel 'start' systems, Res1-Cdc10 and Res2-Cdc10, the former of which plays a major role in mitotic cycle whereas the latter in meiotic cycle."
Control of S-phase periodic transcription in the fission yeast mitotic cycle.
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res2p has a role in switching off S-phase transcription during G2 of the mitotic cycle; the DSC1 bandshift activity requires res2p and correlates with inactive transcription.
"We show that the presence of the in vitro bandshift activity DSC1, conventionally thought to represent the active complex, requires res2p and correlates with inactive transcription."
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S-phase transcription is controlled by both activation and repression, with res2p repressing in G2 as part of DSC1.
"We suggest that S-phase transcription is controlled by both activation and repression, and that res2p represses transcription in G2 of the cell cycle as a part of the DSC1 complex."
res2 deep research (falcon literature synthesis)
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Res2 acts in the nucleus on chromatin, in Res1-Res2-Cdc10 complexes in vegetative cells and a Res2-Cdc10-centred complex in meiosis.
"Res2 acts primarily in the **nucleus on chromatin**, in complexes containing Cdc10 and Res1 during vegetative growth and a Res2–Cdc10-centered complex during meiotic differentiation."
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Full-length Res2 binds DNA weakly on its own and Cdc10 strongly enhances binding; the N-terminal 152 residues mediate homodimerization.
"Full-length Res2 binds DNA only weakly by itself; Cdc10 strongly enhances binding."
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Most MBF-dependent transcripts are constitutively derepressed in res2 cells, but a few (yox1, cig2, mik1) are not, so Res2 is a context-dependent activator/repressor.
"Most MBF-dependent transcripts become constitutively derepressed in *res2Δ*, supporting a major role in transcriptional shutoff, but **yox1, cig2, and mik1** can retain near-wild-type expression."
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Checkpoint kinases act on Cdc10, Nrm1 and Yox1; no direct checkpoint phosphorylation of Res2 has been shown.
"These pathways regulate the Res2-containing complex but have not been shown to modify Res2 directly."