Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The aminoacyl-tRNA synthetases of Drosophila melanogaster.
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MetRS-m (CG31322) is the mitochondrial MetRS, ortholog of MARS2.
"Fly MetRS-m was identified in a screen for genes required for neuronal survival and function."
Mutations in the mitochondrial methionyl-tRNA synthetase cause a neurodegenerative phenotype in flies and a recessive ataxia (ARSAL) in humans.
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Aats-met (MetRS-m) is the fly mitochondrial MetRS, 44% identical to MARS2; fly and human cDNAs rescue lethality of mutant alleles.
"The fly and human cDNAs rescued the lethality associated with FB/Df and HV/Df, the strongest allelic combinations."
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Mutant mitochondria show reduced Complex I activity, consistent with loss of mitochondrial translation.
"We observed a significant decrease in Complex I activity"
Glial lipid droplets and ROS induced by mitochondrial defects promote neurodegeneration.
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Aats-met mutant clones accumulate glial lipid droplets, an indirect consequence of mitochondrial dysfunction.
"Lastly, Aats-met is the Drosophila homolog of mitochondrial methionyl-tRNA synthase 2, or MARS2."
UniProt record Q9VFL5 (SYMM_DROME)