dsbE / ccmG (P0AA86, b2195) notes

Deep research status

• 2026-10-23: just deep-research-falcon ECOLI dsbE was attempted and failed with
  Exception: Failed to authenticate; no other provider credentials were available.
  Review based on cached publications and the UniProt record.

Biology

• Membrane-anchored, periplasmic thioredoxin domain, WCPTC active site (C80/C83)
  PMID:9537397
• Null mutant lacks all c-type cytochromes; cysteine mutants rescued by thiols
  PMID:9537397
  PMID:9537397
• Reduced by DsbD (nDsbD) by direct disulfide exchange
  PMID:16004871
• Acts late, after CcmF/CcmH; not bypassed by dsbA/dsbB mutation or chemical reductant
  PMID:11256948
• Weak reductant (E0' ~ -0.175 to -0.186 V)
  PMID:11350062
  PMID:11843181
• Ortholog biochemistry (R. capsulatus)
  PMID:28634234

Curation decisions

• protein binding (IPI, DsbD): REMOVE per protein-binding policy.
• GO:0015036 and GO:0016491 rows: MODIFY -> GO:0015035 protein-disulfide reductase activity.
• GO:0017004 rows: MODIFY -> GO:0018063 cytochrome c-heme linkage (descendant of GO:0017004).
• Locations plasma membrane and periplasmic space: ACCEPT.
• Fetched PMIDs 11256948, 11350062, 11843181 (UniProt RX lines).

2026-11-06 system I comparative reassessment

Provides periplasmic protein-disulfide reducing activity to the cytochrome c maturation pathway. The E. coli evidence is distinguished from heterologous ortholog assays and engineered expression constructs.

Specific reassessment: Provides periplasmic protein-disulfide reducing activity to the cytochrome c maturation pathway. Exact physiological thiol-exchange partners vary among characterized systems.

PMID:11843181 directly reports weak insulin reduction by leaderless E. coli DsbE; PMID:28634234 directly assays R. capsulatus apocytochrome/CcmH disulfides. The exact relay is not silently transferred between species.

Default Falcon was genuinely attempted and failed with HTTP403 authentication; no provider report was created. Manual literature assessment is recorded here. Original seed rows (including inherited NEW), raw UniProt/GOA, and existing provider files are preserved against original-members-baseline.json. Focused validation and helper history are recorded in the original-panel audit ledger.