fasn-1 (F32H2.5; UniProt P91871) -- manual literature synthesis
Manual
fasn-1 (F32H2.5; UniProt P91871) -- manual literature synthesis
No deep-research API was available for this review. This summary was compiled by hand
from PubMed searches (fasn-1[Title/Abstract] OR F32H2.5[Title/Abstract] AND elegans,
28 hits) and the cached publications in publications/. Each claim carries its source.
Identity and family
- F32H2.5 is the single predicted fatty acid synthase gene of C. elegans; a shorter
homolog, F32H2.6, encodes only an N-terminal portion
PMID:15340492.
- FASN-1 is orthologous to human FASN
PMID:21178429;
emb-14 was renamed fasn-1 after its g43ts lesion was mapped to this gene
PMID:32550489.
- Domain architecture (UniProt P91871, InterPro/Pfam/SMART): KS (PF00109/PF02801),
MAT (PF00698, SMART PKS_AT), DH (PROSITE PS52019), pseudo-KR (PF21149), ER
(SMART PKS_ER), KR (PF08659), ACP (PF00550, PROSITE PS50075 with
pantetheine-phosphoserine Ser2248) and thioesterase (PF00975). This is the
canonical animal type I FAS order.
- Own alignment to human FASN: all annotated catalytic residues (KS Cys/His/His, MAT
Ser, DH His/Asp, ACP Ser, TE Ser/His) are conserved
[file:worm/fasn-1/fasn-1-bioinformatics/RESULTS.md].
Biochemical function
- No purified-enzyme assay of C. elegans FASN-1 was found. Evidence for activity is
(i) orthology and full catalytic-residue conservation, and (ii) in vivo fatty-acid
profiling: transgenic rescue/overexpression raised palmitate and stearate
PMID:21178429.
- RNAi of fasn-1 reduces triglyceride production
PMID:21524356.
- FASN-1 output also feeds mmBCFA/sphingolipid precursors per the anchor-cell study,
which describes "11-methyldodecanoic acid, which is a core precursor for C. elegans
sphingolipids" as a key output of POD-2 and FASN-1 PMID:39007804. Kniazeva et al.
however found RNAi of fasn-1 did not change FA composition selectively
PMID:15340492.
Localization
- Endogenously tagged FASN-1::mNG is cytoplasmic in the anchor cell
PMID:39007804.
Phenotypes (necessity, not activity)
- Loss of function is lethal or causes growth arrest
PMID:15340492.
- Temperature-sensitive alleles g43ts (A1425T / reported elsewhere as A1424T) and g14ts
(G1830R) cause embryonic lethality and permeable eggshells PMID:32550489; loss of
ptr-6 suppresses this PMID:42400361.
- Partial loss-of-function fr8 (M241I) derepresses the antimicrobial peptide reporter
nlp-29 in the epidermis, acting upstream of WNK-1/GCK-3 PMID:21178429.
- Molting and cuticle defects on RNAi PMID:21524356; anchor-cell invasion defects on
AC-specific RNAi PMID:39007804; sensitivity to biguanide-induced reductive stress
[PMID:41394642, preprint].
- These are downstream consequences of fatty-acid supply; none identifies an activity of
FASN-1 beyond fatty acid synthesis.
Regulation