Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa.
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Candidate PvdS-regulated genes were mutated and pyoverdine production measured; the paper named new pvd genes.
"The candidate genes were mutated and the effects of the mutations on pyoverdine production were determined."
Molecular basis of pyoverdine siderophore recycling in Pseudomonas aeruginosa.
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PvdRT-OpmQ, a tripartite ABC efflux system, moves PVD from the periplasm to the medium and recycles PVD after FpvA uptake.
"we identified a tripartite ATP-binding cassette efflux transporter, here named PvdRT-OpmQ, which translocates PVD from the periplasmic space to the extracellular milieu"
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pvdR, pvdT and opmQ mutants each accumulate PVD in the periplasm, complemented by the operon in trans.
"Conversely, PA2389 , PA2390 , and opmQ mutants accumulated five- to sixfold higher amounts of PVD than wild-type PAO1, suggesting an involvement of these genes in PVD export."
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The system does not contribute to antibiotic resistance.
"the PvdRT-OpmQ efflux system does not provide any contribution to P. aeruginosa resistance to antibiotics belonging to the classes of tetracyclines, aminoglycosides, β-lactams and macrolides, and chloramphenicol"
An efflux pump is involved in secretion of newly synthesized siderophore by Pseudomonas aeruginosa.
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Mutants lacking PvdRT-OpmQ accumulate newly synthesised PVD in the periplasm.
"Bacteria lacking this efflux pump accumulated PVD, or a fluorescent precursor, in the periplasm, due to their inability to efficiently secrete into the media newly synthesized PVD."
The PvdRT-OpmQ efflux pump controls the metal selectivity of the iron uptake pathway mediated by the siderophore pyoverdine in Pseudomonas aeruginosa.
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PvdRT-OpmQ secretes new and recycled PVD from the periplasm.
"PvdRT-OpmQ is an ATP-dependent efflux pump involved in the secretion of newly synthesized pyoverdine (PVD) and of PVD that has transported and released its iron into the bacterium from the periplasm into the extracellular medium."
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The pump exports PVD complexed with non-iron metals.
"the PvdRT-OpmQ efflux pump exports unwanted metals complexed with PVD from the bacterium"
An efflux pump is required for siderophore recycling by Pseudomonas aeruginosa.
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pvdRT-opmQ mutation blocks recycling of PVD from the periplasm.
"Mutation of genes encoding this efflux pump (named PvdRT-OpmQ) prevented recycling of PVDI from the periplasm into the extracellular medium."