Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins.
-
PTSG_07215 (SrSeptin2) is a Group 4 (Cdc12-family) septin; PTSG_06009 is Group 1B and PTSG_04106 is Group 1A.
"with animal and fungal septins in Groups 1A (mammalian Septin 3 family), 1B (mammalian Septin 6 family), and 4 (S. cerevisiae Cdc12 family), respectively"
-
Tagged SrSeptin2 is enriched at the basal pole of single cells and rosette cells.
"Strikingly, mTFP1-SrSeptin2 was enriched at the basal poles of single and rosettes cells (Figure 4, B and E) and at points of contact between adjacent cells in rosettes (Figure 4E)."
-
A role for septins in rosette development is a hypothesis, not a finding.
"a family of paralogous genes hypothesized to contribute to multicellular development in S. rosetta"
UniProt record for SrSeptin2 (F2UEE2)
-
SrSeptin2 has a septin-type G domain (residues 21-294) with G1, G3 and G4 motifs.
"PROSITE; PS51719; G_SEPTIN; 1."
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods