Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of intracellular localizations of expressed fusion proteins in living cells
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells.
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Genome-wide protein-complementation screen for telomere-signaling regulators; the THG1L-TERF1 association is one of >300 hits and is not functionally characterized.
"Of the ∼12,000 human proteins examined, we identified over 300 proteins that associated with the six core telomeric proteins."
tRNA(His) guanylyltransferase (THG1), a unique 3'-5' nucleotidyl transferase, shares unexpected structural homology with canonical 5'-3' DNA polymerases.
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Crystal structure and biochemical characterization of the human enzyme establish the core 3'-5' guanylyl-transfer activity, a two-metal-ion (Mg2+) mechanism, GTP/ATP usage, and homotetrameric assembly.
"The 2.3-Å crystal structure of human THG1 (hTHG1) reported here shows that, despite the lack of sequence similarity, hTHG1 shares unexpected structural homology with canonical 5′-3′ DNA polymerases and adenylyl/guanylyl cyclases, two enzyme families known to use a two-metal-ion mechanism for catalysis."
Next-generation sequencing to generate interactome datasets.
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Stitch-seq interactome pipeline; source of a THG1L self-interaction record consistent with homotetramerization.
"We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset."
IHG-1 increases mitochondrial fusion and bioenergetic function.
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IHG-1 (THG1L) acts as a guanine-nucleotide exchange factor for MFN2 (and probably MFN1), promoting mitochondrial fusion and protecting cells from ROS-induced apoptosis; mitochondrial localization is required for the MFN interaction.
"IHG-1 forms complexes with known mediators of mitochondrial fusion-mitofusins (Mfns) 1 and 2-and enhances the GTP-binding capacity of Mfn2, suggesting that IHG-1 acts as a guanine nucleotide exchange factor."
A proteome-scale map of the human interactome network.
A reference map of the human binary protein interactome.
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HuRI binary reference interactome; source of THG1L interactions with LNX1, NTAQ1 and PLEKHF2.
"A reference map of the human binary protein interactome."
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
THG1L transfers GMP to 5' end of tRNA(His)
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Reactome reaction describing THG1L-catalyzed GMP transfer to the tRNA(His) 5' end, the basis for the TAS tRNA-modification/cytosol annotations.
"THG1L transfers GMP to 5' end of tRNA(His)"