Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Falcon deep research report on vanA (Q88GI6, PP_3736) in Pseudomonas putida KT2440
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"VanA (Q88GI6; PP_3736)** is the **terminal oxygenase** subunit responsible for substrate hydroxylation/oxidative demethylation chemistry"
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"the two-component **VanAB** vanillate O-demethylase system that catalyzes oxidative demethylation of vanillate to protocatechuate, releasing **formaldehyde** as a coproduct"
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"VanAB belongs to the **Rieske non-heme iron monooxygenase**"
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"the oxygenase (VanA-family) contains a **Rieske [2Fe–2S] cluster** and a **non-heme iron** catalytic center"
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"VanAB enables growth on vanillate as a sole carbon/energy source by converting it to protocatechuate"
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"VanA operates in the **upper funneling pathway for lignin-derived guaiacyl aromatics**"
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"The product **protocatechuate** feeds into the **β-ketoadipate/pca pathway**"
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"The best-supported physiological substrate in KT2440 is **vanillate**"
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"no in vivo 3MGA O-demethylation despite in vitro activity toward 3MGA"
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"O-demethylation generates **formaldehyde**, which is toxic and must be detoxified/assimilated"
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"the most defensible annotation from current evidence is **intracellular/cytosolic aromatic catabolism**"
vanA literature notes
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"VanA is the oxygenase/Rieske subunit of the two-component VanAB vanillate O-demethylase in KT2440."
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"VanAB-overexpressing KT2440 strains showed faster vanillate utilization"
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"syringate was O-demethylated to gallate by VanAB"
Evolution and engineering of pathways for aromatic O-demethylation in Pseudomonas putida KT2440
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"The native Rieske non-heme iron monooxygenase (VanAB)"
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"those in VanB, the reductase for vanillate O-demethylase"
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"approximately 5x faster vanillate consumption than the"
Metabolism of syringyl lignin-derived compounds in Pseudomonas putida enables convergent production of 2-pyrone-4,6-dicarboxylic acid
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"syringate was found to be O-demethylated to gallate by VanAB, a two-component monooxygenase"
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"the specificity (kcat/KM) of VanAB for syringate was within 25% that for vanillate"
Biotransformation of corn bran derived ferulic acid to vanillic acid using engineered Pseudomonas putida KT2440
Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
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"The specific growth rates on benzoate, p-coumarate, and 4-hydroxybenzoate were considerably higher"
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"vanillin, vanillate, 4-hydroxybenzoate, p-coumarate, benzoate, and ferulate"
Eliminating a global regulator of carbon catabolite repression enhances the conversion of aromatic lignin monomers to muconate in Pseudomonas putida KT2440
OpenScientist PSEPK hydroxycinnamate and vanillate catabolism synthesis
Hydroxycinnamate and vanillate catabolism module