Characterization of an arginine:pyruvate transaminase in arginine catabolism of Pseudomonas aeruginosa PAO1.
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Purified AruH converts L-arginine and pyruvate to 2-ketoarginine and L-alanine.
"High-performance liquid chromatography and mass spectrometry (MS) analyses were employed to detect the presence of the transamination products 2-ketoarginine and L-alanine, thus demonstrating the proposed biochemical reaction catalyzed by AruH"
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Broad amino-donor specificity.
"it has a novel substrate specificity with an order of preference of Arg > Lys > Met > Leu > Orn > Gln"
Functional genomics enables identification of genes of the arginine transaminase pathway in Pseudomonas aeruginosa.
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aruH knockout abolishes the arginine-inducible arginine:pyruvate transaminase activity and arginine utilization in an aruF background.
"This ATase activity was abolished when an aruH knockout was introduced into the strain."
UniProt entry Q9HUI9 (ARUH_PSEAE)
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods