This guide separates repository cache availability from actual source inspection. The review retains 68 imported source objects from 69 raw contexts and nine official products, and adds one separately supported dynactin-binding annotation. Source identity, qualifiers and cached full-text flags are unchanged. Exact quoted support is confined to three core entries; this guide provides locators and access limits, not substitute primary evidence.
| Reference | Effective normal cache | Actual access and scope |
|---|---|---|
| PMID:11375975(https://doi.org/10.1074/jbc.M011005200) | abstract only | Separately recovered original PDF; selected Methods/Results/Discussion read. Human BPAG1 fragments, beta4/BP180 and ERBIN binding; GST lysate assay is not two purified full-length proteins. |
| PMID:11751855(https://doi.org/10.1074/jbc.M109209200) | abstract only | Complete authentic abstract; bounded full-original access failed. Diverse cytolinker isoforms; no invented full Methods or current-nine-product equivalence. |
| PMID:12482924(https://doi.org/10.1242/jcs.00241) | abstract only | Complete authentic abstract; full-original attempts failed. Positive BP230/BP180 cytoplasmic interaction and assembly hierarchy. Independent PMID:10637308 supplies direct support; no unseen Methods claim. |
| PMID:14581450(https://doi.org/10.1083/jcb.200306075) | full | Original selected operative Methods/Results inspected. Human cDNA/library versus native mouse assays distinguished; positive vesicle immunoEM and transport/adaptor evidence; historical long n4 sequence not authenticated. |
| PMID:17043677(https://doi.org/10.1038/sj.mp.4001880) | abstract only | Complete authentic abstract plus literal curated target interactions. DISC1 fragment Y2H; no full-original target Methods claim. |
| PMID:19403692(https://doi.org/10.1091/mbc.e09-01-0051) | full | Normal full cache; selected complete operative Results/Methods read. Human BPAG1e knockdown, actin/polarity/directional persistence, unchanged average speed, Rac1/cofilin rescue; no intrinsic GEF claim. |
| PMID:19632184(https://doi.org/10.1016/j.cell.2009.04.065) | abstract only | Authentic abstract; curated target interactions and original deposited 3GJO structure independently examined. Actual 30-aa entity sequence maps three official DST products despite historical accession label. Full paper not recovered. |
| PMID:19932097(https://doi.org/10.1016/j.yexcr.2009.11.010) | abstract only | Complete authentic abstract; full-original attempts failed. Positive Z-disc and N-terminal plectin/ACTN2 findings; H-zone and exact homodimer assay remain unresolved. |
| PMID:21423176(https://doi.org/10.1038/ncb2216) | full | Normal full cache; selected original Methods read, target supplement row unavailable. Human fibroblast FA isolation/MS; genuine supplemental ZIP lacks target table. No fabricated target counts or contamination conclusion. |
| PMID:21630459(https://doi.org/10.1002/pmic.201000799) | abstract only | Normal abstract only; genuine author-thesis Methods and target table independently read. Human sperm-nuclear preparation; target Q03001 1.4% coverage/3 peptides at physical PDF198, visually inspected. No product-specific peptide claim. |
| PMID:21911577(https://doi.org/10.1074/mcp.M111.012187) | abstract only | Separately recovered genuine original HTML; selected Methods/Results read. Controlled viral fragment/human-library Y2H; target partner is NS5; no target-specific orthogonal validation claimed. |
| PMID:22995871(https://doi.org/10.1038/embor.2012.140) | abstract only | Separately recovered genuine original HTML; selected Methods/Results/Discussion read. Tail/EB1/MT and calcium perturbation; exact construct species and full product mapping unresolved without SI. MT cosedimentation increase not statistically significant. |
| PMID:23414517(https://doi.org/10.1186/2044-5040-3-3) | full | Normal full cache; selected original Methods and target context read. Human muscle library Y2H, PBS and PLA protocol. Large nested Methods paragraph was only partially displayed; no claim of complete body read. |
| PMID:2461961(https://doi.org/10.1172/JCI113803) | abstract only | Complete authentic abstract; scanned full-original route not recovered. Historical basement-membrane-zone terminology; later direct BP230 evidence refines the cellular compartment. |
| PMID:26496610(https://doi.org/10.1016/j.cell.2015.09.053) | abstract only | Complete authentic abstract plus literal curated target records. Human and mouse EB1 partners distinguished in HeLa co-complex assay; original full Methods not read. |
| PMID:28514442(https://doi.org/10.1038/nature22366) | full | Normal full cache; selected Methods read. Tagged human ORFs and HEK293T affinity-purification MS; no purified direct interface. |
| PMID:31413325(https://doi.org/10.1038/s41597-019-0152-0) | full | Normal full cache; selected Methods/data-record scope read. New human-brain Y2H screen with fragment/autoactivation controls; exact DST–DISC1 fragment record retained. |
| PMID:33961781(https://doi.org/10.1016/j.cell.2021.04.011) | full | Normal full flag retained; separately recovered genuine original Methods read. 293T/HCT116 tagged ORF AP-MS. Separate original access is distinguished from a potentially truncated normal representation. |
| PMID:35271311(https://doi.org/10.1126/science.abi6983) | full | Normal cache plus separately recovered author VOR PDF text, selected main Methods read. Endogenous mNG tagging/AP-MS; exact two MAPRE1→DST accession groups independently extracted from the public raw CSV. No isoform assignment. |
| PMID:8575775(https://doi.org/10.1006/geno.1995.9936) | abstract only | Complete authentic abstract; full original not independently read. Human neural-specific exons and mouse/human conservation; inherited epithelial TAS location independently corroborated. |
| PMID:10428034(https://doi.org/10.1016/s0092-8674(00)81017-x) | abstract only | Genuine new normal abstract-only cache; complete abstract read. Mouse neuronal MT stabilization and transport; no claim to independently read full assay Methods. |
| PMID:10637308(https://doi.org/10.1091/mbc.11.1.277) | full | Original selected operative Methods/Results inspected. Human epithelial BP230/BP180/beta4 fragment interaction and localization scope; proposed Golgi interception unmeasured. |
| PMID:14576348(https://doi.org/10.1242/jcs.00764) | abstract only | Genuine new normal abstract-only cache; complete abstract read. C2C12 antibody/N-terminal fusion stress-fiber/nuclear evidence; historical product numbering preserved. |
| PMID:18638474(https://doi.org/10.1016/j.yexcr.2008.06.021) | abstract only | Genuine new normal abstract-only cache; complete abstract read. Mouse sensory-neuron ER/NE pool and transmembrane dystonin-a2 fusion; no exact current human product mapping. |
60ca7eec8412c8fb83772605dc6401d26eaaf6cc4af5acd5365e2fda3ea40d41.a3928145aa1e94f82f272a8f37befeb3d701582fc9fe9cb10f885438de5fc5a6. The separately attempted JCC-named route returned HTML and is not evidence.The PTHR23169 tree, family alignment and ancestral IBD file give the literal evidence path. The source manifest pins exact snapshots. The human leaf PTN000747957 descends from both cited nodes PTN002753757 and PTN002753862; all nine IBDs are reproduced. Mouse Dst/Q91ZU6, MACF1, plectin and epiplakin remain separate donors. A short donor list or the target’s own experimental grounding is not a propagation failure.
The Reactome event R-HSA-2213192 contains the BPAG1e:Plectin input, whose DST-3 component is in cytosol. The embedded Q03001-3 record retains 7570-residue coordinates, whereas the current official product is 2649 residues. The target-resolved modeled location is used; those coordinates are not used for sequence reconstruction or an independently measured soluble pool.
The public replay script and instructions recompute exact interaction joins, two OpenCell accession groups, the peptide/product matches and PAINT lineage from genuine saved inputs. Its hashes and source URLs make the analyzed versions explicit. ROGID matches authenticate database reference sequences; assayed fragment features still govern experimental scope. Positive generic binding without a justified narrower subclass follows the task-specific CLINGEN retention policy.
| Claim | Original location and positive evidence | Limits |
|---|---|---|
| Dynactin binding and transport adaptor | PMID:14581450(https://doi.org/10.1083/jcb.200306075), Results/Figures 2–5 and cloning/immunoprecipitation Methods: human brain-derived ERM/p150 constructs; native mouse p150, p50 and dynein intermediate-chain recovery; transport perturbations. | COS-7/CHO are hosts. Lysate overlay is not purified binding; native localization and transport are mouse. No full current natural product is assigned. |
| NS5 partner class | PMID:21911577(https://pmc.ncbi.nlm.nih.gov/articles/PMC3237087/), viral-library construction, interaction-screening and pairwise-retest Methods: sequence checks, quadruplicate retesting, independent ADE2/HIS3 selection and promiscuity filtering. | No DST-specific split-luciferase or infection phenotype is claimed. The separately recovered full original does not change the normal cache flag. |
| Keratinocyte migration | PMID:19403692(https://doi.org/10.1091/mbc.e09-01-0051), human keratinocyte culture, single-cell motility Methods and Figure 6 Results: reduced persistence, excessive turning, unchanged average speed and Rac1/cofilin bypass. | The cell-type refinement is not a general speed increase or direct locomotor/GEF activity. |
The literal NS5–DST records are IM-21138-183 (EBI-8829260; NS5 feature 56–267), IM-21138-184 (EBI-8829275; 70–130), IM-21138-185 (EBI-8829290; 1–368) and IM-21138-186 (EBI-8829305; 1–835). They identify processed NS5 in P29991 and human-library DST fragments. These are curated sufficient-binding-region features; neither they nor a reference ROGID establish complete experimental clone sequences. The public replay retains the complete accession, taxon, host and feature fields.
Current official definitions distinguish cytoskeletal adaptor activity from dynactin binding: neither is an ancestor of the other. The former retains the integrated transport role, while the latter records the demonstrated contact with the DCTN1/p150 partner and dynactin-containing complexes. Dynactin binding is a child of broad cytoskeletal protein binding; the existing broad replacements concern other partners, so their source rows are not repurposed. Keratinocyte migration is a typed descendant of cell migration and the original cell motility term, and is distinct from maintenance of cell polarity. RNA polymerase binding covers a polymerase molecule or complex, without requiring a host polymerase or catalytic-site contact.