Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
High-throughput phenotypic characterization of Pseudomonas aeruginosa membrane transport genes.
-
Tn5 mutants in each bra operon gene grow less well on alanine, and some also on L-valine and L-isoleucine. The PA1073 mutant fails to grow on L-alanine and grows less well on D-alanine and L-isoleucine.
"In this work, mutants for each of these genes were found to be defective in Alanine metabolism, while utilization of L-Valine and L-Isoleucine was also altered in some cases"
Solubilization and reconstitution of the Pseudomonas aeruginosa high affinity branched-chain amino acid transport system.
-
Reconstituted BraCDEFG imports leucine in an ATP-dependent way, needs all five components, and carries alanine and threonine as well.
"In this reconstituted system, leucine transport was completely dependent on the presence of all five Bra components and on ATP loaded internally to the proteoliposomes."
Transport systems for branched-chain amino acids in Pseudomonas aeruginosa.
Purification and properties of a binding protein for branched-chain amino acids in Pseudomonas aeruginosa.
Cloning, nucleotide sequences, and identification of products of the Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity branched-chain amino acid transport system.
Genetic analysis of the Pseudomonas aeruginosa PAO high-affinity branched-chain amino acid transport system by use of plasmids carrying the bra genes.