Applied the annotation-reviewer and core-function-synthesizer criteria to the genuine human seed and independently reviewed primary-source consultations. All 68 original QuickGO contexts map one-to-one onto 68 stored review rows, preserving original order, evidence, partners and flags. Decisions are 17 ACCEPT, 23 KEEP_AS_NON_CORE, 10 MODIFY, 13 UNDECIDED, 3 TOO_GENERAL and2 MARK_AS_OVER_ANNOTATED. Two unchanged natural products and two substrate-specific plasma-membrane cores are retained; no NEW annotation or supporting-text quotation is added.
Choline and ethanolamine transport are supported by distinct direct cellular, structural and reconstitution evidence. Purified choline uptake is not relabeled as purified ethanolamine flux; kinase-mediated trapping and downstream phosphorylation remain separate. The full-length FLVCR1a scope is not transferred to the 279-residue mitochondrial product. Older measured cellular heme export and product-dependent mitochondrial/erythroid evidence remain contextual, while heme biosynthesis lacks an identified synthetic step performed by FLVCR1. PMID:38693265(https://pubmed.ncbi.nlm.nih.gov/38693265/), PMID:38778100(https://pubmed.ncbi.nlm.nih.gov/38778100/), PMID:15369674(https://pubmed.ncbi.nlm.nih.gov/15369674/), PMID:23187127(https://pubmed.ncbi.nlm.nih.gov/23187127/)
Eight quantified original PDZ-domain/peptide contexts receive MODIFY GO:0030165. Thirteen below-threshold source-pair assertions remain UNDECIDED; this is not a negative-binding conclusion. The HPX interaction remains generic non-core with rabbit reagent and unresolved fragment-insert-species limits. The explicit project instructions retain supported generic interactions where no evidence-backed subtype is established and prohibit new quotations. Published campaign instructions
Exactly three schema-supported original singleton extensions omitted by the seeder are restored from original QuickGO JSON: raw 26/raw 39 part_of GO:0008654 and raw 34 part_of GO:0097037. The typed projection proof explicitly allows only these additions; no original source assertion is silently corrected. All other non-review seed fields and both product objects are exact.
Nine existing main sources were hydrated and hash-checked before genuine fetch. Six absent publications and two absent Reactome summaries were fetched normally; all 17 qualified normal sources are present and all existing bytes are preserved. PMID:15369674 is serialized through this writer so the later FLVCR2 lane can reuse its actual-main cache. Existing PTHR10924 family sources and the already verified tree/MSA/IBD replay are reused; no bulk archive or new environment is created. Known provider credential/quota failures were not retried, and manual research is documented honestly.
The final PMID:38693265 full text was genuinely acquired into the normal cache; selected original Results and Methods were read, preserving the earlier private access records as provenance. PMID:39306721 remains abstract-only in the normal cache, with selected final publisher Methods/Results recovered in the independent consultation. PMID:22483575 detailed Methods and PMID:40972734 exact target assay remain unavailable. No figure-pixel or separate supplement audit is claimed. Evidence synthesis, source/context guide.
Scoped validation and final independent canonical peer review follow this scientific authoring. Their actual outcomes will be appended here.
Schema, reference and ontology checks passed with one expected advisory for the supported HPX generic-binding context under the explicit campaign instruction. Strict GOA matching, report-only status, scaffolded history validation and private rendering passed. Deterministic status remains DRAFT. The PMID helper matched no pathway files and is not claimed to validate these notes. A dependency deprecation warning is unrelated to the biological decisions. Rendered text, counts and representative links were inspected; no pixel-level inspection is claimed.
Independent annotation-reviewer QA read all 68 decisions, the description, both cores and products, the source-specific reference assessments and supporting documents, and found no blocking scientific issue. Original source objects/order and both natural products are preserved, with only the three authorized original annotation extensions restored. The source guide explicitly records the PMID20610401 Methods/Results fragment range of 132–201 versus the Discussion/cache range of 132–301 without claiming a resolved construct sequence. All 17 normal sources, 51 old scratch files and 10 prior branch pins remain unchanged; no local environment was created.
The thirteen below-threshold PMID:36115835 partner assertions retain UNDECIDED and now carry OTHER / INTACT / SUSPECTED / CHECK source-record flags. These are queries for reconciliation, not confirmed errors, evidence-code corrections or negative-binding findings; no report has been filed upstream. The source guide contains a generated table of all 68 measured domain rows for the 21 existing partner accessions, including exact normalized BI, composite pKd, original spreadsheet row, assay threshold, singlicate count and data URL. The split remains eight supported specific-binding refinements and thirteen unresolved pairs.
Both affected HPX/heme-binding rationales and the PMID:20610401 reference note now state the Methods/Results 132–201 versus Discussion/cache 132–301 discrepancy inline. The cached publication remains unchanged. The phospholipid-biosynthesis rationale explicitly identifies transport-mediated precursor entry coupled to labeled downstream precursors, distinguished from handling already completed heme; no unique or obligatorily committed transport route, mature-lipid tracing or biosynthetic catalysis is inferred. Both mitochondrial-membrane refinements acknowledge that GO:0031966 already has retained source rows. The guide also scopes the actual modern heme counter-data without changing reference correctness states or declaring the older cellular results false.
All 68 source objects/order, three original extensions, actions and replacements, both products and cores, the approved description, all 21 reference identities and 17 normal sources are preserved. The previous history and journal entries remain intact. A new explicit-codex EDIT record links PR5716. Affected validation and the final focused canonical peer follow; no new annotation, quotation or gene-completion credit is introduced.
Affected schema/reference/ontology validation passed with the same one intentional HPX generic-binding advisory. New history validation, private rendering and whitespace checks passed. Strict GOA and deterministic DRAFT-status results were reused because all source fields, extensions, actions, replacements and status inputs are unchanged. The earlier PMID helper matched no pathway files and is not claimed to validate notes. The final focused peer read all 18 changed reviews, the reference caveat, complete document additions and derived table, and replayed exact proposal/source conservation with no finding. The rendered table contains all 21 partner rows; rendered text and representative links were inspected without a pixel-level claim.