PA1242 (SprP, Q9I496) review notes

Review session 2026-11-05 (PseudoCAP IBA-seed protocol, tier 2)

Fetched with just fetch-gene PSEAE Q9I496 --alias PA1242; accession matches the worklist. No
deep-research provider was used. Both SprP papers are cached with full text (PMC4618607, PMC3937732).

Literature

PMID:24376018
Catalytic triad E262, H299, S499 (Glu in place of the usual Asp, which explains the UniProt CAUTION
on missing conserved residues).
PMID:26175208; purified
SprP hydrolyses resorufin-casein, inhibited by AEBSF and TPCK, not by E-64, pepstatin A or EDTA;
PMID:26175208.
Deletion mutant: PMID:24376018;
biofilm sixfold up, pyoverdine fivefold up, both complemented; 218 genes changed in the transcriptome.

Annotation decisions

GO:0004252 (IBA, IDA seed, IEA), GO:0006508 (IBA, IEA): ACCEPT.
GO:0008233 (IDA), GO:0008236 (IEA): TOO_GENERAL (entailed by GO:0004252).
Motility IMP rows (GO:0043107, GO:0071977, GO:0071978): MARK_AS_OVER_ANNOTATED; pleiotropic
deletion phenotype, SprP is not motility machinery.
GO:1900232 (IMP): KEEP_AS_NON_CORE. GO:1900377 (IMP): MODIFY to GO:1900705 negative regulation of
siderophore biosynthetic process (pyoverdine was measured).
Action counts: ACCEPT 5, TOO_GENERAL 2, MARK_AS_OVER_ANNOTATED 3, KEEP_AS_NON_CORE 1, MODIFY 1.
No source defects.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN002459139	GO:0004252 serine-type endopeptidase activity	3	90 (31 taxa)	GO:0004252, IDA, PMID:26175208 (PseudoCAP)	SOUND	Full text read; purified enzyme, casein substrate, serine-protease inhibitor profile, S499A inactive. Node term suits a subtilase clade.	B. subtilis AprE P04189, Plasmodium SUB1 Q8I430	Yes

The node's GO:0006508 proteolysis IBD (seeds P04189, P16396, Q8I430) is not seeded by PA1242.