Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Cloning of a human gene, PIG-F, a component of glycosylphosphatidylinositol anchor biosynthesis, by a novel expression cloning strategy.
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Original expression cloning of human PIG-F, identified as a GPI-anchor biosynthesis component acting at the EtNP-transfer step on the three-mannose GPI intermediate.
"PIG-F takes a part in the step of transfer of ethanolamine phosphate to the GPI intermediate containing three residues of mannose."
GPI7 is the second partner of PIG-F and involved in modification of glycosylphosphatidylinositol.
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hGPI7 (PIGG) forms a complex with and is stabilized by PIG-F, which also binds and stabilizes PIG-O; PIGO and PIGG compete for the shared PIG-F stabilizer.
"hGPI7 was associated with and stabilized by PIG-F, which is known to bind to and stabilize PIG-O, a protein homologous to hGPI7."
Biosynthesis and biology of mammalian GPI-anchored proteins.
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PIGO and PIGG are the catalytic EtNP transferases (to Man3 and Man2 respectively) and both are stabilized by association with PIGF; GPI biosynthesis occurs on the ER membrane.
"Both PIGO and PIGG are stabilized by association with PIGF"
A reference map of the human binary protein interactome.
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Source of the HuRI high-throughput binary (Y2H) interaction dataset from which the PIGF 'protein binding' IPI annotations to unrelated membrane proteins derive.
"we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'"
PIGF deficiency causes a phenotype overlapping with DOORS syndrome.
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A homozygous PIGF missense variant (p.Pro172Arg) causes impaired GPI biosynthesis (shown by flow cytometry) and a DOORS-syndrome-overlapping inherited GPI-deficiency phenotype.
"We demonstrate impaired glycosylphosphatidylinositol (GPI) biosynthesis through flow cytometry analysis."
(ethanolamineP) mannose (a1-2) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI -> (ethanolamineP) mannose (a1-2) (ethanolamineP) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI
UniProt entry Q07326 (PIGF_HUMAN)
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UniProt describes PIGF as the stabilizing subunit of the EtNP transferase 2 and 3 complexes (with PIGG and PIGO), an ER membrane multi-pass protein, required to stabilize PIGG and PIGO.
"Stabilizing subunit of the ethanolamine phosphate transferase"