ACO1 (YLR304C, P19414) notes

• Mitochondrial aconitase, EC 4.2.1.3, [4Fe-4S] [UniProt:P19414 "Catalyzes the isomerization of citrate to isocitrate via cis-"].
• Cloned by complementing glu1-1; deletion gives glutamate auxotrophy PMID:1972545.
• Small cytosolic pool serves glyoxylate shunt PMID:15975908.
• Minor homocitrate-dehydratase contribution to lysine synthesis; ACO2 is the main enzyme PMID:23106124.
• mtDNA nucleoid component, binds ds/ssDNA, required for mtDNA maintenance independent of catalysis PMID:15692048; PMID:17698960.
• RIP-chip: binds mitochondrially encoded transcripts PMID:18959479.

Curation decisions: Reactome TOM40:TOM70 import reaction gives spurious IMS (REMOVE) and cytosol TAS (over-annotated). YeastPathways glyoxylate-cycle rows for ACO1 are supported (cytosolic pool). Core: aconitate hydratase in TCA cycle (matrix) + glyoxylate cycle (cytosol).