Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The sonic hedgehog receptor patched associates with caveolin-1 in cholesterol-rich microdomains of the plasma membrane.
Immunohistochemical analysis of Sonic hedgehog signalling in normal human urinary tract development.
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Evidence for allosteric interactions of antagonist binding to the smoothened receptor.
The mammalian Cos2 homolog Kif7 plays an essential role in modulating Hh signal transduction during development.
Identification and mechanism of action of the acylguanidine MRT-83, a novel potent Smoothened antagonist.
Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling.
miR-338-3p suppresses invasion of liver cancer cell by targeting smoothened.
The hedgehog receptor patched is involved in cholesterol transport.
A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened.
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Endogenous SMO accumulates in primary cilia in response to agonist, with BBSome/LZTFL1 controlling its ciliary trafficking.
"In control siRNA transfected cells, SMO localizes to the cilia in response to SMO agonist (SAG) but not in SAG-untreated cells"
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
Structural basis of Smoothened regulation by its extracellular domains.
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Human SMO contains a cholesterol-binding site in its extracellular cysteine-rich domain, and mutations that disrupt cholesterol binding impair native Hedgehog signal transmission.
"Unexpectedly, we find a cholesterol molecule bound to Smoothened in the CRD binding site."
Zfp423 Regulates Sonic Hedgehog Signaling via Primary Cilium Function.
Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric G(i).
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Oxysterol-bound human SMO directly couples to heterotrimeric Gi and activates Gi-dependent Hedgehog signaling.
"We present a cryo-electron microscopy structure of human SMO bound to 24(S),25-epoxycholesterol and coupled to a heterotrimeric Gi protein."
Bi-allelic Variations of SMO in Humans Cause a Broad Spectrum of Developmental Anomalies Due to Abnormal Hedgehog Signaling.
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Biallelic human SMO loss-of-function variants disrupt primary-cilium pathway dynamics and cause multisystem developmental anomalies.
"Cells derived from affected individuals showed normal ciliogenesis but severely altered Hh-signal transduction as a result of either altered PC trafficking or abnormal activation of the pathway downstream of SMO."
Sterols in an intramolecular channel of Smoothened mediate Hedgehog signaling.
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Sterols occupy connected binding sites within SMO and regulate its active signaling conformation.
"These data indicate that sterol transport through the core of SMO is a major regulator of SMO-mediated signaling."
A PKA inhibitor motif within SMOOTHENED controls Hedgehog signal transduction.
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The SMO PKI pseudosubstrate motif directly inhibits PKA catalytic activity and is required for Hedgehog signal transduction.
"SMO uses a decoy substrate sequence to physically block the active site of the cAMP-dependent protein kinase (PKA) catalytic subunit (PKA-C) and extinguish its enzymatic activity."
GRK2 kinases in the primary cilium initiate SMOOTHENED-PKA signaling in the Hedgehog cascade.
Activating Smoothened mutations in sporadic basal-cell carcinoma.
Characterization of two patched receptors for the vertebrate hedgehog protein family.
PTCH1 inhibits accumulation of SMO in the primary cilium in the absence of Hh signal
SMO translocates to the cilium
CSNK1A1 and ADRBK1 dissociate from p-SMO dimer
UniProtKB reviewed record for human SMO (Q99835)
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SMO is a sterol-activated GPCR that signals through Gi and direct PKA-C sequestration at plasma and ciliary membranes.
"G protein-coupled receptor, which transduces the smoothened signaling pathway"