Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Hypothetical gene C18orf42 encodes a novel protein kinase A-binding protein.
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AKAIN1 binds specifically the type II regulatory subunits of PKA.
"Immunoprecipitation and pull-down assays showed that C18orf42 binds specifically to the type II regulatory subunits of PKA."
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Expressed AKAIN1 blocks AKAP-mediated subcellular localization of PKA.
"that of C18orf42 could block the AKAP-mediated subcellular localization of PKA."
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The authors propose it as an endogenous disruptor of PKA-AKAP interactions - a proposal, not a demonstrated physiological role.
"may be a novel PKA signaling gene that serves as an endogenous disruptor peptide for PKA-AKAP interactions."
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Expression is preferentially neural.
"C18orf42 is expressed preferentially in neural tissues."
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The endogenous-protein experiments used a FLAG knock-in in a mouse cell line, which is why the mouse ortholog carries IDA annotations from this paper.
"incorporate the FLAG tag into the C-terminus of the endogenous C18orf42 protein in the mouse neural cell line Neuro2a."
A reference map of the human binary protein interactome.
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HuRI is a proteome-scale binary interactome, the source of the generic protein-binding row.
"Here we present a human 'all-by-all'"
The Inflammatory Gene PYCARD of the Entorhinal Cortex as an Early Diagnostic Target for Alzheimer's Disease.
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AKAIN1 is among ten genes differentially expressed in Alzheimer's disease entorhinal cortex - an expression observation consistent with neural expression, with no functional content.
"including ten significant genes (TRMT2B, SLC22A2, SEMA3E, PRORY, AKAIN1, NIT1, ITGB2-AS1, FGF14-AS2, PYCARD, ADIRF)"
UniProtKB P0CW23 (AKAI1_HUMAN) flat file
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UniProt records the binding partners as PRKAR2A and PRKAR2B.
"specifically with RII-regulatory subunits of PKA (PRKAR2A and PRKAR2B)"
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The RII-binding site is predicted, not shown, to form an amphipathic helix.
"DOMAIN: The RII-alpha binding site, predicted to form an amphipathic"
Affinage mechanistic annotation for AKAIN1 (human)
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The affinage summary agrees with the review on RII binding and neural expression, and adds nothing beyond the one paper.
"AKAIN1 (C18orf42) is a neural-tissue-enriched protein that engages the cAMP-dependent protein kinase A (PKA) signaling system through direct binding to PKA regulatory subunits"