GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

file:PSEPK/PP_2483/PP_2483-uniprot.txt

UniProtKB entry for PP_2483

UniProt records Q88K10 in PTHR43777:SF1 with the imported molybdenum-cofactor cytidylyltransferase label.

"DR PANTHER; PTHR43777; MOLYBDENUM COFACTOR CYTIDYLYLTRANSFERASE; 1. DR PANTHER; PTHR43777:SF1; MOLYBDENUM COFACTOR CYTIDYLYLTRANSFERASE; 1."

file:PSEPK/PP_2483/PP_2483-goa.tsv

QuickGO GOA annotations for PP_2483

The fetched table contains the one broad annotation reviewed here.

"UniProtKB Q88K10 PP_2483 enables GO:0016779 nucleotidyltransferase activity molecular_function ECO:0000256 IEA GO_REF:0000117 ARBA:ARBA00026258 160488 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) UniProt MobA-like NTP transferase domain-containing protein 20260706"

file:PSEPK/PP_2483/PP_2483-deep-research-openscientist.md

OpenScientist gene research for PP_2483

The report explicitly acknowledges that PP_2483 has no target-specific experimental support.

"**No direct experimental data** exist for PP_2483; the annotation is inferred from orthology, conserved domains, genomic context, and characterized homologs. Q88K10 is an unreviewed TrEMBL entry."
The report incorrectly promotes the unresolved PP_2482 paralog to canonical MoaA.

"| **PP_2482** | **MoaA / GTP 3′,8-cyclase** (K03639, EC 4.1.99.22) | First committed step of Moco biosynthesis |"
The report overstates indirect neighborhood evidence as direct evidence for dedicated client delivery.

"This parallel genomic architecture is direct evidence for **dedicated, acceptor-specific MCD delivery**"
The report's sequence values differ from the saved local comparison and do not support a specific MocA assignment.

"Needleman-Wunsch global identity: PP_2483 is actually closer to *E. coli* **MobA** (34.3%) than to *E. coli* **MocA** (25.3%), while *E. coli* MobA vs MocA share only 24.2%, and PP_2483 vs its paralog PP_4230 = 48.1%."

file:interpro/panther/PTHR43777/PTHR43777-entries.csv

PANTHER representative entries for the MocA-containing family

The local PANTHER representatives include reviewed E. coli MocA Q46810 in PTHR43777:SF1.

"Q46810,Molybdenum cofactor cytidylyltransferase,protein,83333,Escherichia coli (strain K12),Escherichia coli (strain K12),mocA,192,PTHR43777:SF1,MOLYBDENUM COFACTOR CYTIDYLYLTRANSFERASE,True"

file:PSEPK/moaA/moaA-bioinformatics/RESULTS.md

Reproducible sequence comparison of KT2440 MoaA-family and MobA-like proteins

The saved comparisons place PP_2483 closer to E. coli MobA but do not establish nucleotide specificity.

"- PP_2483 is more similar to the E. coli MobA control (39.29%) than to the E. coli MocA control (28.49%), whereas PP_4230 is more similar to MocA (39.62%) than to MobA (28.74%). PP_2483 and PP_4230 are 48.13% identical to one another. - The generated KEGG link table maps both PP_2483 and PP_4230 to K07141. It maps the reviewed E. coli controls to distinct MobA (K03752) and MocA (K07141) orthologies, respectively."

file:PSEPK/moaA/moaA-bioinformatics/mob_like_kegg_orthology.tsv

KEGG gene-to-orthology links for KT2440 MobA-like candidates and reviewed controls

The reproducible KEGG lookup maps PP_2483 to MocA orthology K07141.

"PP_2483 Q88K10 ppu:PP_2483 ko:K07141"