Gene Ontology annotation through association of InterPro records with GO terms

Annotation inferences using phylogenetic trees

Combined Automated Annotation using Multiple IEA Methods

Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.

phzM and phzS insertion mutants of P. aeruginosa lack pyocyanin.

"P. aeruginosa with insertionally inactivated phzM or phzS developed pyocyanin-deficient phenotypes."

PMID:16946471

The purification, crystallization and preliminary structural characterization of PhzM, a phenazine-modifying methyltransferase from Pseudomonas aeruginosa.

PhzM crystallizes with one dimer in the asymmetric unit.

"They contain one dimer in the asymmetric unit"

PMID:17253782

Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.

Purified PhzM alone does not methylate PCA; with PhzS, NADH and O2, pyocyanin is formed in a SAM-dependent way, and a transient PhzM-PhzS encounter is required.

"Pyocyanin formation required PhzM, PhzS, PCA, SAM, oxygen, and NADH consistent with a methyl transfer reaction and a flavin-mediated hydroxylation."
PhzM is a homodimer with a plant O-methyltransferase-like intertwined interface.

"Structural alignments with known methyltransferases show that PhzM is most similar to the plant O-methyltransferases"

PMID:22562990

Catabolite repression control of pyocyanin biosynthesis at an intersection of primary and secondary metabolism in Pseudomonas aeruginosa.

file:PSEAE/phzM/phzM-uniprot.txt

UniProt entry Q9HWH2 (PHZM_PSEAE)

file:PSEAE/phzM/phzM-bioinformatics/RESULTS.md

Composition of PAINT node PTN002463933 (PTHR43712) and its leaf IBAs