Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB reviewed entry for ilvC
QuickGO GOA annotations for ilvC
PANTHER family metadata for ilvC
Falcon (Edison Scientific) deep research report for P. putida KT2440 ilvC (Q88DZ0 / PP_4678)
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"Ketol-acid reductoisomerase (KARI; IlvC/AHAIR)** is a conserved bacterial enzyme family within the 6-phosphogluconate dehydrogenase (6PGDH)-type superfamily that performs a chemically coupled **alkyl-migration (isomerization)** and **NADP(H)-dependent reduction** step in BCAA biosynthesis."
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"IlvC/AHAIR is the **second** enzyme in the pyruvate-to-2-ketoisovalerate segment (AHAS → AHAIR/IlvC → DHAD/IlvD)."
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"IlvC requires **NADPH** for the reduction half-reaction and a **divalent metal ion (most commonly Mg²⁺)** to support the alkyl-migration/isomerization chemistry, which is mechanistically integrated in a single catalytic cycle for most KARIs."
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"a Gram-negative bacterial IlvC homolog was reported as a **soluble protein** that could be purified without detergents, consistent with a **cytosolic enzyme** (as expected for a central-metabolism biosynthetic enzyme operating on cytosolic intermediates)."
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"A genome-wide knockout screen on glucose minimal medium identified **ilvC (PP4678)** among genes whose disruption prevents growth on M9 minimal medium, i.e., conditionally essential in that environment."
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"*ilvC* mutants showed branched-chain amino-acid auxotrophy, with reported requirements involving isoleucine and valine/leucine supplementation depending on the assay context."
OpenScientist literature and database synthesis for PSEPK IlvC
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Confirms Q88DZ0 as the ketol-acid reductoisomerase used at the shared second step of branched-chain amino-acid biosynthesis.
"The gene **_ilvC_** (ordered locus **PP_4678**; UniProt **Q88DZ0**) of *Pseudomonas putida* strain KT2440 (ATCC 47054 / DSM 6125 / NCIMB 11950) encodes **ketol-acid reductoisomerase (KARI)**"
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No direct Q88DZ0 experiment was found; target-specific kinetics, structure, localization, and regulation therefore remain unverified.
"No direct experimental characterization of the *P. putida* KT2440 enzyme."