Identification of QuiP, the product of gene PA1032, as the second acyl-homoserine lactone acylase of Pseudomonas aeruginosa PAO1.
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quiP transposon mutants cannot grow on decanoyl-HSL as sole carbon source; complementation restores growth.
"Transposon mutants of quiP were defective for growth when P. aeruginosa was cultured in medium containing decanoyl-HSL as a sole carbon and energy source."
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E. coli expressing quiP gains long-chain AHL acylase activity, releasing HSL.
"The results confirm that quiP encodes a protein with acyl-HSL acylase activity."
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Periplasmic location is predicted from the signal sequence.
"PA1032 is predicted to be secreted into the periplasm based on its signal sequence"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt