Generated by analyze_adamtsl3.py. Every number below is recomputed on each run from primary sources (PANTHER PAINT GAFs, UniProt, QuickGO, IntAct); nothing is hardcoded.
Node-level annotations come from PANTHER's IBD.gaf; the leaf column entries come from gene_association.paint_uniprot.gaf.gz, i.e. the projections PAINT actually exports, not a reconstruction of them.
Curated node annotations in this family:
| node | GO id | evidence | negated | taxon |
|---|---|---|---|---|
| PTN000347317 | GO:0004222 metalloendopeptidase activity | IBD | no | taxon: |
| PTN000347317 | GO:0006508 proteolysis | IBD | no | taxon: |
| PTN000347317 | GO:0030198 extracellular matrix organization | IBD | no | taxon: |
| PTN000347317 | GO:0031012 extracellular matrix | IBD | no | taxon: |
| PTN002673039 | GO:0004222 metalloendopeptidase activity | IKR | yes | taxon:117571 |
| PTN002673039 | GO:0006508 proteolysis | IRD | yes | taxon:117571 |
Which of those four terms each family member actually receives:
| member | accession | GO:0031012 extracellular matrix |
GO:0030198 extracellular matrix organization |
GO:0004222 metalloendopeptidase activity |
GO:0006508 proteolysis |
|---|---|---|---|---|---|
| ADAMTS1 | Q9UHI8 | is_active_in | involved_in | enables | involved_in |
| ADAMTS9 | Q9P2N4 | is_active_in | involved_in | enables | involved_in |
| ADAMTS10 | Q9H324 | is_active_in | involved_in | enables | involved_in |
| ADAMTS17 | Q8TE56 | is_active_in | involved_in | enables | involved_in |
| ADAMTSL1 | Q8N6G6 | - | - | - | - |
| ADAMTSL2 | Q86TH1 | is_active_in | involved_in | NOT | - |
| ADAMTSL3 | P82987 | is_active_in | - | - | - |
| ADAMTSL4 | Q6UY14 | is_active_in | involved_in | - | - |
| ADAMTSL5 | Q6ZMM2 | is_active_in | - | - | - |
| PAPLN | O95428 | - | - | - | - |
| THSD4 | Q6ZMP0 | is_active_in | involved_in | - | - |
| Adamtsl1_mouse | Q8BLI0 | is_active_in | - | - | - |
| Adamtsl2_mouse | Q7TSK7 | is_active_in | involved_in | NOT | - |
| Adamtsl3_mouse | G3UXC7 | is_active_in | - | - | - |
| Adamtsl4_mouse | Q80T21 | is_active_in | involved_in | - | - |
| Papln_mouse | Q9EPX2 | - | - | - | - |
| Thsd4_mouse | Q3UTY6 | is_active_in | involved_in | - | - |
| madd-4_worm | P90884 | - | - | - | - |
NOT GO:0004222: ADAMTSL2, Adamtsl2_mouse.GO:0004222: ADAMTSL1, ADAMTSL3, ADAMTSL4, ADAMTSL5, PAPLN, THSD4, Adamtsl1_mouse, Adamtsl3_mouse, Adamtsl4_mouse, Papln_mouse, Thsd4_mouse, madd-4_worm.GO:0004222: none.GO:0004222, so absence in the ADAMTSL rows is a property of the projection and not of the query.GO:0031012 carries 17 WITH/FROM tokens: 16 protein donors (0 self-referential) plus PANTHER tree nodes. 16 of 16 carry their own experimental annotation to GO:0031012 or a descendant.
The specific terms those donors hold experimentally: GO:0001527, GO:0005604, GO:0005614, GO:0031012 - i.e. the donor set is heterogeneous in which matrix compartment it occupies, so the general parent is the least common ancestor rather than a curator failing to be specific.
| token | resolves to | reviewed | own experimental evidence for the term |
|---|---|---|---|
| FB:FBgn0003137 [2 candidates] | Q868Z9 Ppn (Drosophila melanogaster) | Swiss-Prot | GO:0005604 IDA (PMID:11076767), GO:0005604 IDA (PMID:3320045) |
| FB:FBgn0032252 [4 candidates] | Q9VKV3 loh (Drosophila melanogaster) | TrEMBL | GO:0031012 IDA (PMID:23874219) |
| MGI:MGI:109249 [5 candidates] | P97857 Adamts1 (Mus musculus) | Swiss-Prot | GO:0005604 IDA (PMID:16061471), GO:0031012 IDA (PMID:12907688), GO:0031012 IDA (PMID:9593739) |
| MGI:MGI:1347346 [5 candidates] | Q68SA9 Adamts7 (Mus musculus) | Swiss-Prot | GO:0031012 IDA (PMID:15192113) |
| MGI:MGI:1347356 [4 candidates] | Q8C9W3 Adamts2 (Mus musculus) | Swiss-Prot | GO:0031012 IDA (PMID:16556917) |
| MGI:MGI:1913798 [4 candidates] | D3Z689 Adamtsl5 (Mus musculus) | TrEMBL | GO:0031012 HDA (PMID:22159717), GO:0031012 IDA (PMID:23010571) |
| MGI:MGI:1925044 [4 candidates] | Q7TSK7 Adamtsl2 (Mus musculus) | Swiss-Prot | GO:0031012 IDA (PMID:17509843) |
| MGI:MGI:2386139 [3 candidates] | Q9EPX2 Papln (Mus musculus) | Swiss-Prot | GO:0031012 HDA (PMID:22159717), GO:0005604 IDA (PMID:18757743) |
| MGI:MGI:2389008 [3 candidates] | Q80T21 Adamtsl4 (Mus musculus) | Swiss-Prot | GO:0005614 IDA (PMID:18757743), GO:0031012 IDA (PMID:18757743) |
| MGI:MGI:2672033 [4 candidates] | Q3UTY6 Thsd4 (Mus musculus) | Swiss-Prot | GO:0001527 IDA (PMID:19940141), GO:0031012 IDA (PMID:19940141) |
| PANTHER:PTN000347317 | PANTHER tree node PTN000347317 (not a protein) | - | - |
| RGD:621241 [3 candidates] | Q9WUQ1 Adamts1 (Rattus norvegicus) | Swiss-Prot | GO:0031012 IDA (PMID:15777654) |
| UniProtKB:Q6ZMM2 | Q6ZMM2 ADAMTSL5 (Homo sapiens) | Swiss-Prot | GO:0031012 IDA (PMID:23010571) |
| UniProtKB:Q8TE56 | Q8TE56 ADAMTS17 (Homo sapiens) | Swiss-Prot | GO:0031012 IDA (PMID:28176809) |
| UniProtKB:Q9H324 | Q9H324 ADAMTS10 (Homo sapiens) | Swiss-Prot | GO:0031012 IDA (PMID:21402694) |
| UniProtKB:Q9P2N4 | Q9P2N4 ADAMTS9 (Homo sapiens) | Swiss-Prot | GO:0031012 IDA (PMID:12514189) |
| WB:WBGene00003242 | O76840 mig-6 (Caenorhabditis elegans) | Swiss-Prot | GO:0005604 IDA (PMID:19297413) |
GO:0005515 partner set15 IPI rows over 13 distinct partners. Gene-level IntAct detection-method counts for P82987: anti tag coip x15, proximity-dependent biotin identification x1, two hybrid array x16, two hybrid prey pooling approach x16, validated two hybrid x16.
That gene-level tally must not be read as evidence about these 13 pairs. It also covers partners that are not in GOA. Disaggregating per pair is what answers the question, and it is done below.
Every partner resolves to a reviewed Swiss-Prot entry at canonical length: True (no TrEMBL/ORFeome substitutions).
ADAMTSL3 itself has 30 distinct IntAct partners; the median for its 13 annotated partners is 188.
| partner | length | protein name | UniProt subcellular location | IntAct methods for THIS pair | distinct IntAct partners |
|---|---|---|---|---|---|
| CYSRT1 (A8MQ03) | 144 aa | Cysteine-rich tail protein 1 | Cornified envelope | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 516 |
| MDFI (Q99750) | 246 aa | MyoD family inhibitor | Nucleus; Cytoplasm | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 483 |
| KRT40 (Q6A162) | 431 aa | Keratin, type I cytoskeletal 40 | not annotated | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 448 |
| KRTAP10-8 (P60410) | 259 aa | Keratin-associated protein 10-8 | not annotated | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 415 |
| NOTCH2NLA (Q7Z3S9) | 236 aa | Notch homolog 2 N-terminal-like protein A | Secreted; Cytoplasm | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 276 |
| GLRX3 (O76003) | 335 aa | Glutaredoxin-3 | Cytoplasm, cytosol; Cytoplasm, cell cortex; Cytoplasm, myofibril, sarcomere, Z line | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 190 |
| KRTAP1-1 (Q07627) | 177 aa | Keratin-associated protein 1-1 | not annotated | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 188 |
| KRTAP12-3 (P60328) | 96 aa | Keratin-associated protein 12-3 | not annotated | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 141 |
| KRTAP2-4 (Q9BYR9) | 128 aa | Keratin-associated protein 2-4 | not annotated | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 89 |
| KRTAP3-2 (Q9BYR7) | 98 aa | Keratin-associated protein 3-2 | not annotated | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 84 |
| KRTAP10-6 (P60371) | 365 aa | Keratin-associated protein 10-6 | not annotated | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 77 |
| KRTAP5-7 (Q6L8G8) | 165 aa | Keratin-associated protein 5-7 | not annotated | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 61 |
| KRTAP2-3 (P0C7H8) | 128 aa | Keratin-associated protein 2-3 | not annotated | two hybrid array; two hybrid prey pooling approach; validated two hybrid | 10 |
GOA partners with any non-Y2H detection method: 0 of 13 - every one of these pairs rests on yeast two-hybrid sub-methods alone, with no orthogonal assay.
For contrast, the 17 IntAct partners that are not in GOA are where the other methods live: anti tag coip, proximity-dependent biotin identification, two hybrid array, two hybrid prey pooling approach, validated two hybrid. These are separate publications and are out of scope for this review, but they are why the gene-level method counter cannot settle the question.
GOA rows: 18. Reviewed rows derived from GOA: 18. Reviewer-proposed (NEW) rows: 4. Coverage OK: True.
Actions: ACCEPT x2, KEEP_AS_NON_CORE x1, MARK_AS_OVER_ANNOTATED x15, NEW x4.