Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Coupled enzymatic production of sulfite, thiosulfate, and hydrogen sulfide from sulfur: purification and properties of a sulfur oxygenase reductase from the facultatively anaerobic archaebacterium Desulfurolobus ambivalens.
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SOR purified from aerobically grown cells; oxygen-dependent simultaneous production of sulfite, thiosulfate and H2S from sulfur; thiosulfate largely non-enzymatic.
"In the presence of oxygen but not under a hydrogen atmosphere, the enzyme simultaneously produced sulfite, thiosulfate, and hydrogen sulfide from sulfur."
Molecular characterization of the sor gene, which encodes the sulfur oxygenase/reductase of the thermoacidophilic Archaeum Desulfurolobus ambivalens.
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Cloning and sequencing of sor; transcript strongly induced under aerobic growth.
"The transcript of the sor gene in aerobically grown cells was amplified 38- to 42-fold relative to that in anaerobically grown cells."
The sulphur oxygenase reductase from Acidianus ambivalens is a multimeric protein containing a low-potential mononuclear non-haem iron centre.
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SOR is the initial enzyme of the sulfur-oxidation pathway; contains one mononuclear non-haem iron per subunit; 24-mer hollow sphere.
"Iron was found in the wild-type enzyme at a stoichiometry of one iron atom/subunit."
Identification of core active site residues of the sulfur oxygenase reductase from Acidianus ambivalens by site-directed mutagenesis.
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His86, His90 and Glu114 are iron ligands; Cys31 is essential for activity.
"It was concluded that the Fe site with H86, H90 and E114 as ligands and C31 constitute the core active site whereas C101 and C104 optimize reaction conditions."
X-ray Structure of a self-compartmentalizing sulfur cycle metalloenzyme.
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1.7 A structure; 24-mer hollow sphere; mononuclear non-heme iron with 2-His-1-carboxylate facial triad plus cysteine persulfide form the active site.
"A cysteine persulfide and a low-potential mononuclear non-heme iron site ligated by a 2-His-1-carboxylate facial triad in a pocket of each subunit constitute the active sites, accessible from the inside of the sphere."
The sulfur oxygenase reductase from Acidianus ambivalens is an icosatetramer as shown by crystallization and Patterson analysis.
Substrate pathways and mechanisms of inhibition in the sulfur oxygenase reductase of acidianus ambivalens.
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SOR is a cytoplasmic 24-mer and the initial enzyme of sulfur oxidation; pore mutagenesis and inhibitor soaking define substrate pathways and Zn2+ inhibition.
"The spherical, hollow, cytoplasmic enzyme is composed of 24 identical subunits with an active site pocket each comprising a mononuclear non-heme iron site and a cysteine persulfide."
Determinants of sulphur chemolithoautotrophy in the extremely thermoacidophilic Sulfolobales.
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SOR (with TQO) restored sulfur oxidation in S. acidocaldarius; physiological role (first step vs recycling) discussed.
"Initial studies indicated SOR catalyses the essential first step in oxidation of elemental sulphur, but an ancillary role for SOR as a 'recycle' enzyme has also been proposed."
Key role of cysteine residues in catalysis and subcellular localization of sulfur oxygenase-reductase of Acidianus tengchongensis.
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Ortholog in A. tengchongensis found in cytoplasm and associated with the cytoplasmic membrane.
"SOR is not only present in the cytoplasm but also associated with the cytoplasmic membrane of A. tengchongensis."