Gene Ontology annotation through association of InterPro records with GO terms

The DOCK-family InterPro entry supports GEF activity and small-GTPase signaling.

Annotation inferences using phylogenetic trees

Five literal PAINT ancestral assertions lie on the reconstructed DOCK8 target lineage and have experimental descendant grounding.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

Curated UniProt cytoplasmic and membrane locations underlie electronic location mappings.

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

Target-matched HPA ICC/IF data support cytosol and plasma-membrane pools.

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

Mouse Dock8 experimental records support ortholog-derived leading-edge, polarity and migration assertions.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Combined orthology and DOCK-family evidence supports the inherited GEF activity.

PMID:19946888

Defining the membrane proteome of NK cells.

The membrane-proteome study is the source of the curated positive DOCK8 membrane assertion.

PMID:22006977

DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice.

DOCK8-deficient human memory T cells show impaired proliferation under receptor/co-stimulatory stimulation; separate mouse experiments distinguish modest primary expansion effects from severe persistence and recall defects.

PMID:22581261

DOCK8 functions as an adaptor that links TLR-MyD88 signaling to B cell activation.

DOCK8 associates with MyD88/Pyk2 and supports Pyk2 activation and phosphorylation-dependent Src/Lyn recruitment in the TLR9 signaling response of human B cells.

PMID:24255178

Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions.

The curated AP-MS source records support DOCK8 coassociation with LRCH1 and LRCH2 in human cells.

PMID:25416956

A proteome-scale map of the human interactome network.

Binary interaction screening detects DOCK8 with MEOX2, KRT40 and CARHSP1.

PMID:26871637

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.

The isoform-interaction study supports DOCK8-fragment association with PIMREG.

PMID:28028151

LRCH1 interferes with DOCK8-Cdc42-induced T cell migration and ameliorates experimental autoimmune encephalomyelitis.

DOCK8 promotes CDC42 activation, T-cell polarity and migration, while LRCH1 competes for DOCK8 association and phosphorylation relieves this restraint.

PMID:28514442

Architecture of the human interactome defines protein communities and disease networks.

BioPlex AP-MS supports DOCK8–LRCH2 coassociation.

PMID:31515488

Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.

The variant-interaction study preserves a positive DOCK8–LRCH1 Y2H interaction for the recorded LRCH1 variant.

PMID:32203420

Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions.

DOCK8 associates with LRCH2 and undergoes LRCH-dependent redistribution; its tested product has a condition-specific negative cellular activity-screen result.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

BioPlex3 AP-MS supports DOCK8 coassociation with LRCH1 and LRCH2 in human 293T cells.

PMID:40205054

Multimodal cell maps as a foundation for structural and functional genomics.

The primary U2OS AP-MS network contains LRCH1-bait association with DOCK8.

Reactome:R-HSA-9012999

RHO GTPase cycle

DOCK8 occurs in the Rho-GTPase activation network through the RHOJ GEF catalyst set.

Reactome:R-HSA-9017491

RHOJ GEFs activate RHOJ

DOCK8 is included in a cytosolic GEF catalyst set for a modeled RHOJ nucleotide-exchange event.

PMID:22461490

DOCK8 is a Cdc42 activator critical for interstitial dendritic cell migration during immune responses.

Purified DHR2 exchange assays support CDC42 activation, while mouse dendritic-cell experiments establish a role in directed migration and the 3VHL structural entity is mouse.

PMID:25762780

HkRP3 is a microtubule-binding protein regulating lytic granule clustering and NK cell killing.

DOCK8 associates with HkRP3 in human NK-cell systems and participates in polarization and adhesion responses.

PMID:15304341

Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology.

The historical DOCK8 clone was identified in a Cdc42-interaction screen and was observed at cell-edge regions containing lamellipodia.

PMID:22634526

The RhoGEF Zizimin-related acts in the Drosophila cellular immune response via the Rho GTPases Rac2 and Cdc42.

Fly Zir genetically interacts with Rac2 and Cdc42 during cellular immune responses.