fabV (PA2950, Q9HZP8) notes

Triclosan-resistant enoyl-ACP reductase (TER/FabV family). No deep-research provider was
available; literature checked by hand (PMID:19933806 and PMID:27965638 full texts cached;
QuickGO for the V. cholerae and Y. pestis seeds). Consistent with the fabA, fabB and fabD
reviews (fatty acid biosynthetic process accepted as core for FAS II enzymes).

Literature

Zhu et al. 2010 (PMID:19933806): purified PaFabV reduces enoyl-ACP with NADH ["The addition of
PaFabV, PaFabI, or EcFabI plus NADH to the reaction mixture yielded butyryl-ACP"]; also reduces
crotonyl-CoA but with ~30-fold lower Vmax (UniProt kinetics); fabV deletion makes PAO1

2000-fold more triclosan-sensitive.
Huang et al. 2016 (PMID:27965638, full text read): ENR activity in fabV-mutant extracts
["The ENR activity (in μmol/min per mg extract protein) in cell-free extract of PAO170 was
0.175 ± 0.006, and was lower than that in the cell-free extract of wild-type strain PAO1 (0.478
± 0.032)."], partly restored by complementation; slower growth, less AHL, swarming and
virulence factors (indirect phenotypes, not annotated).
Seeds: V. cholerae FabV Q9KRA3 (IDA GO:0004318, PMID:18032386); Y. pestis FabV Q8Z9U1 (IDA
GO:0004318, PMID:22244758).

Review decisions

GO:0004318 (IDA, IMP, IBA, IEA) and GO:0006633 (IDA, IBA, IEA): ACCEPT, core.
GO:0050343 trans-2-enoyl-CoA reductase (all rows): KEEP_AS_NON_CORE (genuine secondary
activity, much lower than on enoyl-ACP).
GO:0051287 NAD binding (all rows): KEEP_AS_NON_CORE (cofactor).
GO:0016491 oxidoreductase activity: TOO_GENERAL (entailed by GO:0004318).

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN002206122	GO:0004318 enoyl-[acyl-carrier-protein] reductase (NADH) activity	3	7 (6 taxa)	GO:0004318, IMP, PMID:27965638 (PseudoCAP)	SOUND	Full text read: ENR activity falls to ~37% in fabV-mutant extracts and rises on complementation. Lysate-activity evidence alone is weak, but the activity is FabV's own (purified-enzyme IDA, PMID:19933806); residual activity is FabI's. Node term suits the FabV clade.	V. cholerae FabV Q9KRA3 (IDA); Y. pestis FabV Q8Z9U1 (IDA); also FabV's own UniProt IDA	Yes, on the V. cholerae and Y. pestis seeds

No PSEUDOCAP or GO_CENTRAL defect.