Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
CrebA regulates secretory activity in the Drosophila salivary gland and epidermis.
The small G protein Arl1 directs the trans-Golgi-specific targeting of the Arf1 exchange factors BIG1 and BIG2.
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Arl1-GTP binds Sec71 directly through the region N-terminal to the Sec7 domain and recruits it to trans-Golgi membranes.
"This indicates that Arl1 recruits Sec71 to Golgi membranes, but it appears that Arl1’s effects are augmented by additional interactions elsewhere in the protein."
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Sec71-GFP localizes to the trans-Golgi in S2 cells.
"Thus, Sec71 binds to Arl1-coated liposomes in vitro and colocalizes with Arl1 on the trans-Golgi in vivo."
Sec71 functions as a GEF for the small GTPase Arf1 to govern dendrite pruning of Drosophila sensory neurons.
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Sec71 is a GEF for Arf1, preferentially binding GDP-Arf1, and is required for dendrite pruning of ddaC neurons.
"We further identified Sec71 as a guanine nucleotide exchange factor for Arf1 that preferentially interacts with its GDP-bound form."
Identification of Genes Required for Apical Protein Trafficking in Drosophila Photoreceptor Cells.
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Sec71 knockdown in photoreceptors reduces apical (Crb, Eys, Rh1) and basolateral (Nrv) proteins, placing Sec71 in Golgi biosynthetic trafficking.
"Taken together, our observations indicate that Sec71 contributes to the secretion of both apical and basolateral proteins in PRCs, suggesting a defect in Golgi processing (Figure 3E)."
Sec71 separates Golgi stacks in Drosophila S2 cells.
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Genome-edited BFA-resistant and BFA-hypersensitive Sec71 alleles show Sec71 is the BFA target for BFA-body formation and controls Golgi stack separation.
"These results indicate that Sec71 is the sole BFA target for BFA body formation and controls Golgi stack separation."
Golgi-dependent reactivation and regeneration of Drosophila quiescent neural stem cells.
Arf1 and ARFGEF2/Sec71 control neuroblast polarity by anchoring nonmuscle myosin II.
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Sec71 loss of function or dominant-negative Sec71 disrupts neuroblast apicobasal polarity and cortical PI(4)P and myosin II localization.
"Taken together, these observations indicate that Arf1 and Sec71 may facilitate the localization of nonmuscle myosin II to the cell cortex in mitotic neuroblasts."
UniProtKB entry Q9VJW1 (Sec71, Drosophila melanogaster)
Sec71 ComplexPortal SEC62-SEC63 complex membership check