Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
The Pseudomonas aeruginosa exotoxin A regulatory gene, ptxS: evidence for negative autoregulation.
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PtxS-dependent binding to a 52-bp ptxS upstream fragment, lost in the ptxS mutant.
"The gel shift band was not detected when the 52-bp fragment was incubated with the lysate of the ptxS isogenic mutant PAO1::ptxS."
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PtxS autoregulates its synthesis.
"PtxS autoregulates its synthesis by binding to a specific sequence within the ptxS upstream region"
Autoregulation of the Pseudomonas aeruginosa protein PtxS occurs through a specific operator site within the ptxS upstream region.
Genes for carbon metabolism and the ToxA virulence factor in Pseudomonas aeruginosa are regulated through molecular interactions of PtxR and PtxS.
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PtxS binds the -10 region of target promoters and occludes RNA polymerase.
"PtxS operates as a repressor that binds to the -10 region of the target promoters and occludes RNA polymerase access"
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PtxS regulates its own promoter and the kgu and gad catabolic operons in response to 2-ketogluconate.
"PtxS with 2-ketogluconate is the regulator of the gluconate degradation pathway and controls expression from its own promoter and also from the P(gad) and P(kgu) for the catabolic operons"
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PtxS binds PtxR with nanomolar affinity; 2-ketogluconate prevents the interaction.
"In vitro, PtxS recognises PtxR with nanomolar affinity, but this interaction does not occur in the presence of 2-ketogluconate, the specific effector of PtxS."
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PtxS does not bind the toxA promoter.
"PtxR binds to the -35 region of the P(toxA) promoter in front of the exotoxin A gene, whereas PtxS does not bind to this promoter"