Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
Expression pattern of Brachyury and Not in the sea urchin: comparative implications for the origins of mesoderm in the basal deuterostomes.
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SpBra is the S. purpuratus orthologue of vertebrate Brachyury.
"SpBra, the orthologue of the vertebrate Brachyury gene"
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Embryonic expression is in the vegetal plate and secondary mesenchyme founder cells and is then extinguished; the gene is re-expressed in the larval vestibule of the adult rudiment and in the hydrocoel mesoderm.
"this gene is expressed during embryogenesis in the embryonic vegetal plate and secondary mesenchyme founder cells, and expression is then extinguished"
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SpBra is expressed in the mesoderm of the left and right hydrocoels but in no larva-specific tissue.
"SpBra is also expressed in the mesoderm of both left and right hydrocoels, and it is not expressed in any larva-specific tissues"
brachyury Target genes in the early sea urchin embryo isolated by differential macroarray screening.
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Brachyury expression in the S. purpuratus vegetal plate is confined to the presumptive endoderm.
"Brachyury expression in the vegetal plate is confined to the presumptive endodermal cells."
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Endodermal Bra-dependent genes include cytoskeletal modulators implicated in gut morphogenesis, consistent with the gastrulation block caused by interfering with Brachyury function.
"Some of the endodermal genes that respond to Brachyury are cytoskeletal modulators that may play a role in gut morphogenesis. This finding is consistent with the block in gastrulation induced by interfering with Brachyury function in sea urchins"
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Brachyury links the endoderm regulatory state to morphogenesis and terminal gut effector genes.
"the brachyury gene transduces information about the state of endodermal specification to genes that modulate morphogenesis and genes that perform terminal functions in the gut"
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The pigment-lineage SMC genes recovered in the screen are judged indirect targets.
"the SMC genes are likely to be indirect targets of Brachyury-induced signaling from the surrounding endoderm to the central mesoderm"
The role of Brachyury (T) during gastrulation movements in the sea urchin Lytechinus variegatus.
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A dominant-repressor LvBrac-Engrailed fusion blocks gastrulation movements without blocking endoderm and mesoderm marker gene expression.
"Microinjection of mRNA encoding this LvBrac-EN construct resulted in a block in gastrulation movements but not expression of endoderm and mesoderm marker genes."
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Brachyury is required for the morphogenetic movements in both the vegetal (blastopore) and oral (stomodeal) expression domains.
"It was then determined that LvBrac is necessary for the morphogenetic movements occurring in both expression regions."
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The oral ectoderm domain refines to the stomodeal opening.
"The second domain of LvBrac expression first appears broadly in the oral ectoderm at mesenchyme blastula stage and at later embryonic stages is refined to just the stomodael opening."
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Vegetal expression depends on autonomous beta-catenin signalling in the macromeres.
"Vegetal LvBrac expression depends on autonomous beta-catenin signaling in macromeres and does not require micromere or veg2-inductive signals."
The endoderm gene regulatory network in sea urchin embryos up to mid-blastula stage.
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brachyury shares the early veg2 endoderm expression pattern with foxA, blimp1b and hox11/13b.
"foxA, blimp1b, hox11/13b and brachyury showed very similar expression patterns."
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Both foxA and brachyury are controlled by Tcf, Hox11/13b and Otx, and ultimately by GataE.
"Both genes appear to be expressed under the control of Tcf, Hox11/13b and Otx, as mentioned above, and are also ultimately affected by GataE."
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Tcf/Groucho repression through Tcf sites restricts brachyury expression to the endoderm precursors.
"Mutation of Tcf binding sites in the cis-regulatory regions of hox11/13b, foxA and brachyury, and eve results in dramatic ectopic expression of reporter constructs in all or most domains of the embryo."
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brachyury morpholino strongly depresses otx-beta transcripts, revealing positive feedback from brachyury into the endoderm kernel.
"perturbation of brachyury expression strongly depressed expression levels of otx–β transcripts"
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Otx is activated by the products of its target genes blimp1b and brachyury.
"Otx is activated by its own gene product as well as the products of its target genes blimp1b and brachyury."
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brachyury morpholino does not affect foxA levels at 18 hpf.
"Injection of a brachyury MASO did not affect the expression levels of foxA at 18 hpf."
A gene regulatory network controlling the embryonic specification of endoderm.
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brachyury shifts from veg2 endoderm at 18 h to the veg1 (hindgut) endoderm progenitors by 24 h.
"By 24 h post-fertilization, hox11/13b and brachyury, which are both expressed in veg2 endoderm at 18 h, are being transcribed instead in veg1 endodermal progenitors, where eve also continues to be expressed"
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Hox11/13b drives brachyury expression in both the veg2 and veg1 endoderm GRNs.
"In both the early veg2 and the later veg1 endoderm GRNs, Hox11/13b functions as a driver of brachyury expression, as shown by the specific reduction of endodermal brachyury expression in embryos injected with hox11/13b MASO"
An ancestral Wnt-Brachyury feedback loop in axial patterning and recruitment of mesoderm-determining target genes.
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Genome-wide Brachyury ChIP-seq was performed in S. purpuratus (and Nematostella).
"we present a genome-wide target-gene screen using chromatin immunoprecipitation sequencing in the sea anemone Nematostella vectensis, an early branching non-bilaterian, and the sea urchin Strongylocentrotus purpuratus"
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Brachyury sits in an ancestral feedback loop with FoxA and canonical Wnt signalling.
"Our analysis reveals an ancestral gene regulatory feedback loop connecting Brachyury, FoxA and canonical Wnt signalling involved in axial patterning"
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The vertebrate mesodermal Brachyury targets are largely absent from the sea urchin target set.
"hardly any of the key mesodermal downstream targets in vertebrates are found in the sea anemone or the sea urchin"
A single-cell RNA-seq analysis of Brachyury-expressing cell clusters suggests a morphogenesis-associated signal center of oral ectoderm in sea urchin embryos.
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Brachyury is expressed in the invaginating endoderm and in the oral ectoderm of the invaginating mouth.
"In sea urchin embryos, Brachyury is expressed in the invaginating endoderm, and in the oral ectoderm of the invaginating mouth opening."
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All three endoderm clusters of the S. purpuratus scRNA-seq atlas express Brachyury in blastulae.
"Our results showed that cells of all three endoderm clusters expressed Brachyury in blastulae."
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Brachyury-positive oral ectoderm cells of the ventral organizer invaginate to form the stomodeum.
"This suggests that the ventral organizer contains Brachyury-positive cells which invaginate to form the stomodeum."
Hedgehog signaling patterns mesoderm in the sea urchin.
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The Hedgehog ligand is expressed by the endoderm downstream of Brachyury and FoxA.
"At gastrulation the Hh ligand is expressed by the endoderm downstream of the Brachyury and FoxA transcription factors in the endomesoderm gene regulatory network."
A sea urchin homologue of the chordate Brachyury (T) gene is expressed in the secondary mesenchyme founder cells.
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The sea urchin Brachyury gene is single copy.
"The HpTa gene is present as a single copy per haploid genome."
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HpTa transcripts mark the SMC founder cells, vegetal plate and the tip of the invaginating archenteron.
"HpTa transcripts were found in the secondary mesenchyme founder cells, vegetal plate of the mesenchyme blastula, extending tip of the invaginating archenteron"
Brachyury homolog (HpTa) is involved in the formation of archenteron and secondary mesenchyme cell differentiation in the sea urchin embryo.
Expression pattern of Brachyury in the embryo of the sea urchin Paracentrotus lividus.
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PlBra is first expressed in a ring at the endoderm-mesoderm border and later around the blastopore and in the stomodeum.
"PlBra is first expressed at the end of cleavage in a ring of cells at the border between the presumptive endoderm and mesoderm territories."