hsp-70 (C12C8.1) curation notes

UniProt O45246 (TrEMBL, 643 aa), WormBase C12C8.1. Inducible cytosolic HSP70
(counterpart of human HSPA1A/B). Deep research: not run (falcon times out in
this environment; perplexity-lite unavailable). Review built from cached
publications and PubMed searches.

Identity

• C. elegans has a multigene hsp70 family PMID:2225768.
• Four cytosolic HSP70s: HSP-1, C12C8.1, F44E5.4, F44E5.5 PMID:41387410.
• Sequence: begins MSTCKAIGIDLG (no signal peptide), ends EEVD (cytosolic HSP70 co-chaperone motif). ARBA "ER lumen" is therefore wrong -> REMOVE.

Expression

• Silent at ambient temperature, strongly induced by heat; C12C8.1 promoter is the standard phsp70::gfp reporter PMID:23637632.

• 1000-fold mRNA induction after heat shock PMID:28198373.

• HSF-1 dependent PMID:27688402.
• Tunicamycin induction, attenuated in xbp-1 (Table I of PMID:12186849) -> ER UPR/IRE1 HEP rows kept as non-core (output gene, cytosolic).

Decisions

• GO:0044183 (IBA, ISS) MODIFY -> GO:0140662 ATP-dependent protein folding chaperone, matching hsp-1.
• GO:0005832 CCT (IDA, PMID:9434769, abstract-only): MARK_AS_OVER_ANNOTATED. Abstract names 52-65 kDa CCT subunits and co-purifying HSP60; an HSP70 is not a CCT subunit. Not removed since full text unread.
• Lifespan IGI (PMID:14668486) KEEP_AS_NON_CORE PMID:14668486.
• No gene-specific biochemistry exists for C12C8.1; MF rests on the PAINT HSP70 node and ISS from HSPA1A.