Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Disruption of the gene encoding the cell adhesion molecule DdCAD-1 leads to aberrant cell sorting and cell-type proportioning during Dictyostelium development.
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Loss of cadA reduces EDTA-sensitive (calcium-dependent) cell adhesion by half and perturbs cell-type proportioning and pattern formation.
"The cadA-null cells showed a 50% reduction in EDTA-sensitive cell adhesion"
Comparison of molecular mechanisms mediating cell contact phenomena in model developmental systems: an exploration of universality.
An activated Ras protein alters cell adhesion by dephosphorylating Dictyostelium DdCAD-1.
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Activated RasG increases DdCAD-1-dependent cohesion and DdCAD-1 cell surface localization while reducing its phosphorylation.
"This increased cohesion was DdCAD-1-dependent and was correlated with increased localization of DdCAD-1 at the cell surface"
Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake.
Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca(2+)-dependent cell-cell adhesion.
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DdCAD-1 has two beta-sandwich domains; the N-terminal domain mediates homophilic binding and the C-terminal domain tethers it to the membrane.
"Whereas the N-terminal domain has a major role in homophilic binding, the C-terminal domain tethers the protein to the cell membrane"
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A Ca2+-bound DdCAD-1 dimer provides the structural basis for DdCAD-1-mediated cell-cell adhesion.
"we propose a model for the Ca(2+)-bound DdCAD-1 dimer as a basis for understanding DdCAD-1-mediated cell-cell adhesion"
Proteomic analysis of Legionella-containing phagosomes isolated from Dictyostelium.
Cell adhesion molecule DdCAD-1 is imported into contractile vacuoles by membrane invagination in a Ca2+- and conformation-dependent manner.
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DdCAD-1 is made as a soluble cytoplasmic protein and imported into contractile vacuoles for transport to the plasma membrane.
"DdCAD-1 is synthesized in the cytoplasm as a soluble protein and then transported by contractile vacuoles to the plasma membrane for surface presentation or secretion"
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Calcium binding is required for import of DdCAD-1 into contractile vacuoles.
"Import of DdCAD-1 was compromised when two of its three Ca(2+)-binding sites were mutated, indicating a role for Ca(2+) in the import process"
The cell adhesion molecule DdCAD-1 regulates morphogenesis through differential spatiotemporal expression in Dictyostelium discoideum.
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Differential distribution of DdCAD-1 on prestalk versus prespore cells governs cell sorting in slugs.
"These results show that DdCAD-1 influences the sorting behavior of cells in slugs by its differential distribution on the prestalk and prespore cells"
Ca(2+) -calmodulin interacts with DdCAD-1 and promotes DdCAD-1 transport by contractile vacuoles in Dictyostelium cells.
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Only Ca2+-bound calmodulin binds DdCAD-1, and calmodulin promotes DdCAD-1 import into contractile vacuoles.
"Co-immunoprecipitation and pull-down studies showed that only Ca(2+) -bound calmodulin was able to bind DdCAD-1"
Dictyostelium lipid droplets host novel proteins.
ATP-Binding Cassette Transporter B4 Anchors the Cell Adhesion Molecule DdCAD-1 to Cell Membrane in Dictyostelium discoideum.
Proteomic profiling of the extracellular matrix (slime sheath) of Dictyostelium discoideum.
Curcumin inhibits development and cell adhesion in Dictyostelium discoideum: Implications for YakA signaling and GST enzyme function.
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Curcumin suppresses expression of the cell adhesion proteins DdCAD and csA, delaying adhesion and development.
"expression of the extracellular matrix/cell adhesion proteins (DdCAD and csA)"
Social amoebae establish a protective interface with their bacterial associates by lectin agglutination.
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Secreted CadA is a calcium-dependent, galactose-specific lectin that agglutinates Gram-negative bacteria and protects amoebae from bacterial toxicity.
"CadA, a cell adhesion protein that functions in D. discoideum development, is also a bacterial agglutinin that forms a protective interface at the plaque edge that limits exposure of vegetative amoebae to bacteria"
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CadA binding to K. pneumoniae is inhibited by galactose, consistent with a lectin recognizing bacterial surface carbohydrate.
"This suggests that CadA is a lectin that binds to carbohydrates on the surface of K. pneumoniae, perhaps to their lipopolysaccharide, which is rich in galactose moieties"
Trafficking of adhesion and aggregation-modulating proteins during the early stages of Dictyostelium development.
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CadA localizes to the cell periphery in growth-phase and starved cells, and its intracellular/extracellular levels are modulated by autophagy and trafficking genes.
"As for CadA, it localizes to the cell periphery in growth-phase and starved cells"
Identification of major proteins associated with Dictyostelium discoideum endocytic vesicles.
Characterization of the cell adhesion molecule gp24 in Dictyostelium discoideum. Mediation of cell-cell adhesion via a Ca(2+)-dependent mechanism.
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gp24 (DdCAD-1) is a cell adhesion molecule that functions through a homophilic, calcium-dependent mechanism and binds calcium.
"these in vitro assays provide direct evidence that gp24 is a cell adhesion molecule that most likely functions through a homophilic mode of interaction"
Molecular cloning and characterization of DdCAD-1, a Ca2+-dependent cell-cell adhesion molecule, in Dictyostelium discoideum.
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DdCAD-1 associates with the ecto-surface of the plasma membrane despite lacking a signal peptide or transmembrane domain.
"immunofluorescence microscopy demonstrates the association of DdCAD-1 with the ecto-surface of the plasma membrane"
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Cell binding activity resides in the N-terminal segment, not the C-terminal Ca2+-binding domain.
"the cell binding activity is dependent on the amino-terminal segment and not the carboxyl-terminal Ca2+-binding domain"
Novel redistribution of the Ca(2+)-dependent cell adhesion molecule DdCAD-1 during development of Dictyostelium discoideum.
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Before aggregation DdCAD-1 relocates from diffuse cytoplasm to the cell periphery and becomes enriched on membrane ruffles, lamellipodia, and filopodia at cell contacts.
"A high concentration of DdCAD-1 also becomes associated with lamellipodia and filopodia, which often appear to participate in cell contact formation"
The cell adhesion molecule DdCAD-1 in Dictyostelium is targeted to the cell surface by a nonclassical transport pathway involving contractile vacuoles.
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DdCAD-1 is transported to the cell surface from the cytosol via contractile vacuoles, a nonclassical secretion pathway.
"DdCAD-1 is transported to the cell surface via contractile vacuoles, which are normally involved in osmoregulation"
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DdCAD-1 preferentially associates with contractile vacuoles and is present both on the surface and in the cytosol.
"Immunofluorescence microscopy and subcellular fractionation revealed a preferential association of DdCAD-1 with contractile vacuoles"