Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Isolation and expression analysis of the isopropylmalate synthase gene family of Arabidopsis thaliana.
-
Cloned the Arabidopsis IPMS-like gene family; two cDNAs complemented an E. coli Leu auxotroph (MAM3 among them per Textor et al. 2007).
"cDNAs of IMS2 and IMS3 lacking the 5' chloroplast leader sequence were able to complement a leucine auxotroph of E. coli."
Glucosinolate and amino acid biosynthesis in Arabidopsis.
-
MAML (MAM3) knockout lacks long-chain aliphatic glucosinolates, restored by complementation.
"A MAML knockout line (KO) lacked long-chain aliphatic GSLs, which were restored when the KO was transformed with a functional MAML gene."
MAM3 catalyzes the formation of all aliphatic glucosinolate chain lengths in Arabidopsis.
-
MAM3 catalyzes all six condensation reactions of Met chain elongation and forms all chain lengths in vivo.
"Here, we describe the biochemical properties of the MAM3 enzyme, which is able to catalyze all six condensation reactions of Met chain elongation that occur in Arabidopsis."
-
MAM3 has low IPMS activity and complements IPMS-deficient E. coli at 28 C but is unlikely to act as an IPMS in planta.
"However, because the specific activity of the IPMSs for 2-oxoisovalerate is at least 40 times higher than those of MAM3, it appears very unlikely that this enzyme is active as IPMS under normal growth conditions"
-
MAM3 is immunolocalized to chloroplasts.
"Immunolocalization experiments with leaf tissue of Arabidopsis (Col-0) performed with the anti-MAM3 serum confirmed that MAM3 is targeted to the chloroplasts"