Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Atp11p and Atp12p are assembly factors for the F(1)-ATPase in human mitochondria.
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Human ATP11 complements yeast atp11.
"Here we report the isolation of the cDNAs and the characterization of the human genes for Atp11p and Atp12p and show that the human proteins function like their yeast counterparts."
Architecture of the human interactome defines protein communities and disease networks.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Multimodal cell maps as a foundation for structural and functional genomics.
Atp11p and Atp12p are chaperones for F(1)-ATPase biogenesis in mitochondria.
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Atp11p and Atp12p are chaperones for F1 alpha/beta assembly.
"Our work, which employs the yeast Saccharomyces cerevisiae as a model system for studies of mitochondrial function, has provided evidence that assembly of the mitochondrial alpha and beta subunits into the F(1) oligomer requires two molecular chaperone proteins called Atp11p and Atp12p."
ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration.
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Atpaf1-KO hearts have less F1 and fewer ATP synthase dimers.
"Western blots and Blue-Native electrophoresis (BN-PAGE) demonstrated a decreased F1 content and ATP synthase dimers in the Atpaf1-KO heart."
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ATPAF1 colocalizes with ATP5B in mitochondria.
"We confirmed that ATPAF1 was co-localized with the mitochondrial β-subunit ATP5B via immunofluorescence staining, which was consistent with previous studies (Saito et al."
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Atpaf1-KO mice are small, with high lactate.
"While the Atpaf2-KO mice were embryonically lethal, Atpaf1-KO mice grew to adulthood but with smaller body sizes and elevated blood lactate later in life."
Differential expression of ATPAF1 and ATPAF2 genes encoding F(1)-ATPase assembly proteins in mouse tissues.
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ATPAF1 is an F1 assembly factor for the beta subunit.
"Atp11p (Atpaf1p; F(1)-ATPase assembly factor 1) and Atp12p (Atpaf2p; F(1)-ATPase assembly factor 2) are proteins required for the assembly of beta (F(1)-beta) and alpha (F(1)-alpha) subunits into the mitochondrial ATPase."
UniProt entry Q5TC12 (ATPF1_HUMAN)
Affinage mechanistic annotation for ATPAF1 (human)