Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Combined Automated Annotation using Multiple IEA Methods
Identification of the aceA gene encoding isocitrate lyase required for the growth of Pseudomonas aeruginosa on acetate, acyclic terpenes and leucine.
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PA2634 (aceA) encodes the ICL; activity is induced by acetate, citronellol and leucine, and the gene is needed for growth on these substrates.
"The isocitrate lyase gene (aceA) corresponding to ORF PA2634 of the PAO1 strain genome was identified in one of these mutants."
Type III secretion system expression in oxygen-limited Pseudomonas aeruginosa cultures is stimulated by isocitrate lyase activity.
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aceA mutants show reduced T3SS expression and enhanced anaerobic biofilm formation, as do other TCA and glyoxylate cycle mutants.
"Biofilm formation under anaerobic conditions was significantly enhanced in the aceA mutant as well as in the other mutants that showed reduced PcrV expression"
UniProt record Q9I0K4 (PA2634)