Gene Ontology annotation through association of InterPro records with GO terms

InterPro domain-based annotations can lead to over-annotation when domain presence does not guarantee the associated function, particularly for domains like Amidase_2 where not all family members are enzymatically active.

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

Keyword-based annotations correctly identify PGRP-LD as involved in immunity, but lack specificity about its regulatory role.

PGRP-LD mediates A. stephensi vector competency by regulating homeostasis of microbiota-induced peritrophic matrix synthesis

PGRP-LD is a non-catalytic PGRP that lacks conserved residues for amidase activity
Functions as a negative regulator of innate immunity
Maintains gut microbiota homeostasis
Required for peritrophic matrix integrity
Knockdown increases Plasmodium susceptibility

PMID:31907025

Gut microbiota is essential in PGRP-LA regulated immune protection against Plasmodium berghei infection

Contextual evidence for PGRP family regulation of immunity and microbiota interactions in Anopheles

file:ANOGA/PGRPLD/PGRPLD-deep-research-falcon.md

Deep research synthesis for PGRPLD

PGRP-LD is a non-catalytic PGRP lacking key residues for amidase activity

"The A. stephensi PGRP-LD lacks most conserved residues required for PGN binding and amidase catalytic activity"
Functions as negative regulator of innate immunity

"Knockdown of PGRP-LD upregulates multiple immune effectors (e.g., cecropin, gambicin, defensin, DUOX), consistent with a negative regulatory role constraining immune activation in response to microbiota"
Transmembrane protein at gut barrier

"Described as a transmembrane PGRP"