Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
Deep research report on mcm-4 (falcon/Edison)
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MCM-4 contributes as one subunit to the ATPase and helicase activities of the MCM2-7/CMG complex, with ATP hydrolysis in the MCM ring driving DNA translocation and unwinding.
"MCM proteins carry ATP-binding motifs and are attributed ATPase and helicase activities, with ATP hydrolysis within the MCM ring driving DNA translocation and unwinding in CMG."
Regulation of DNA synthesis and replication checkpoint activation during C. elegans development.
C. elegans MCM-4 is a general DNA replication and checkpoint component with an epidermis-specific requirement for growth and viability.
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lin-6 corresponds to mcm-4, encoding the C. elegans MCM4 ortholog, a conserved subunit of the MCM2-7 pre-RC and replicative helicase complex.
"lin-6 corresponds to mcm-4 and encodes an evolutionarily conserved component of the MCM2-7 pre-RC and replicative helicase complex."
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mcm-4 is required for postembryonic somatic DNA synthesis and for the replication checkpoint coupling M-phase entry to S-phase completion.
"lin-6 is required for the checkpoint that couples M phase entry to S phase completion"
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MCM-4 is expressed in all dividing cells and associates with chromatin in late anaphase.
"The MCM-4 protein is expressed in all dividing cells during embryonic and postembryonic development and associates with chromatin in late anaphase"
The demethylase NMAD-1 regulates DNA replication and repair in the Caenorhabditis elegans germline.
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NMAD-1 binds MCM-4 directly in vitro (recombinant pulldown); MCM-4 was identified in NMAD-1 IP/MS as a candidate binder, but the NMAD-1/MCM-4 interaction was not confirmed in vivo (only NMAD-1/TOP-2 was confirmed in vivo).
"NMAD-1 directly bound to MTSS-1, TOP-2, and MCM-4, components of the DNA replication machinery"
Isolation and genetic characterization of cell-lineage mutants of the nematode Caenorhabditis elegans.
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Classic screen for postembryonic cell-lineage (lin) mutants, the screen in which lin-6(e1466) - later shown to be mcm-4 - was isolated. The quoted sentence establishes the screen itself; the cached record does not contain the strings 'lin-6' or 'e1466', so the identification of that specific allele is sourced from the other references in this review, not from this one.
"Twenty-four mutants that alter the normally invariant post-embryonic cell lineages of the nematode Caenorhabditis elegans have been isolated and genetically characterized."