PGK1 notes (P00560, YCR012W)
- 3-phosphoglycerate kinase, EC 2.7.2.3 [UniProt:P00560 "Reaction=(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl"]; cloned and shown to make functional enzyme PMID:6254992.
- pgk mutants cannot use glucose PMID:400791; only PGK gene, reversible step also needed for gluconeogenesis.
- Cytosolic PMID:27385335; minor mitochondria-associated pool PMID:16962558. Plasma membrane HDA PMID:16622836 likely contaminant of an abundant protein.
- NOT protein-disulfide reductase (mammalian PGK plasmin reductase) PMID:11130727 — negative annotation accepted.
Decisions: REMOVE bifid shunt; MODIFY obsolete glycolysis term -> GO:0006096; plasma membrane MARK_AS_OVER_ANNOTATED.