NCU03033 evidence notes

NCU03033 is the Hap2/NF-YA subunit of the heterotrimeric CCAAT-binding transcription factor. Its conserved DNA-recognition domain works with the two partner subunits to bind CCAAT promoter elements and regulate nuclear RNA polymerase II transcription.

Identity and provenance

The exact current accession is Q7SGY4; NCU03033 is retained as the current locus identifier. No independently established formal gene-symbol replacement was found. Original claims are preserved in NCU03033-protnlm-source.json from the live API snapshot retrieved 2026-09-09T03:00:51.831347+00:00. The current sequence is not proven to be the prediction-time input, and placeholder API dates do not establish release or training membership.

Primary evidence and justified transfer

• PMID:25589790 "The CBC consists of
  three subunits, which are named in the filamentous fungus Aspergillus nidulans
  HapB, HapC, and HapE."
• PMID:25589790 "Initial
  binding of the CBC to CCAAT boxes is mandatory for DNA recognition of HapX."

The specific domain/family observation used is DR InterPro; IPR001289; NFYA.. Family membership and characterized relatives establish the inference; ARBA or AI-generated names are not independent functional evidence. PAINT is a curator-reviewed ancestral assertion, not donor-count evidence.

Unresolved assertions

No unresolved GOA assertion remains at the reviewed level of specificity. Experimental observations on relatives are distinguished from target observations.

Additional primary evidence: PMID:19965775 "The corresponding Aspergillus nidulans CCAAT-binding factor (AnCF)
consists of the subunits HapB, HapC and HapE. All of the three subunits are
necessary for DNA binding.". Full text also documents prior cross-species complementation evidence for Hap2/HapB/NF-YA, supporting conserved family transfer. Oxidative regulation of the partner HapC is not assigned as a Hap2-specific phenotype.

Falcon report appraisal

The actual Falcon report arrived after the wrapper timeout and was inspected. It supports the conserved Hap2/NF-YA CCAAT-recognition role and cautions against transferring individual fungal regulons, essentiality or autonomous activation-domain function. The present review already grounds the family mechanism in Aspergillus primary biochemical papers (PMID:25589790; PMID:19965775). Its additional cellulase, respiration and iron-control suggestions are not used as target-specific annotations. The wrapper reported a 600-second Falcon timeout and the fallback returned HTTP 401 insufficient quota; the original Falcon child nevertheless completed, so no replacement report was fabricated or duplicate job submitted.